Recombinant Anti-HES5 antibody [EPR15578] (ab194111)
![Western blot - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231823-anti-hes5-antibody-epr15578-western-blot.jpg "Western blot - Anti-HES5 antibody [EPR15578] (ab194111)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15578] to HES5
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-HES5 antibody [EPR15578]
See all HES5 primary antibodies -
Description
Rabbit monoclonal [EPR15578] to HES5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- 293, C6 and PC-12 cell lysates; Human cerebellum, Human fetal brain, mouse brain, mouse kidney, mouse spleen, rat brain, rat kidney and rat spleen lysates; Human glioblastoma, Human adenocarcinoma of endometrium and mouse cerebral cortex tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15578 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
|
|
| IP |
1/40.
|
| Notes |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
|
IP
1/40. |
Target
-
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. -
Tissue specificity
Expressed in fetal heart and brain tumors. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 388585 Human
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- Omim: 607348 Human
- SwissProt: Q5TA89 Human
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 57971 Human
see all -
Alternative names
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
see all
Images
-
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + 293 cell lysate at 10 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231824-anti-hes5-antibody-epr15578-western-blot.jpg)
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + Human cerebellum lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231825-anti-hes5-antibody-epr15578-western-blot.jpg)
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution + Human fetal brain lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231826-anti-hes5-antibody-epr15578-western-blot.jpg)
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)All lanes : Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lane 7 : C6 cell lysate
Lane 8 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231827-anti-hes5-antibody-epr15578-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231828-anti-hes5-antibody-epr15578-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231829-anti-hes5-antibody-epr15578-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-231830-anti-hes5-antibody-epr15578-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HES5 antibody [EPR15578] (ab194111)Western blot detection of HES5 in Human cerebellum cell lysate immunoprecipitated using ab194111 at 1/40 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-HES5 antibody [EPR15578] (ab194111)](https://www.abcam.com/ps/products/194/ab194111/Images/ab194111-9-benefits-of-recombinant-antibodies.png)
Anti-HES5 antibody [EPR15578] (ab194111)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (21)
ab194111 has been referenced in 21 publications.
- Li C et al. MIR4435-2HG in exosomes promotes gastric carcinogenesis by inducing M2 polarization in macrophages. Front Oncol 12:1017745 (2022). PubMed: 36483041
- Xie Q et al. Tspan5 promotes epithelial-mesenchymal transition and tumour metastasis of hepatocellular carcinoma by activating Notch signalling. Mol Oncol 15:3184-3202 (2021). PubMed: 33955149
- Li D et al. African swine fever virus protein MGF-505-7R promotes virulence and pathogenesis by inhibiting JAK1- and JAK2-mediated signaling. J Biol Chem 297:101190 (2021). PubMed: 34517008
- Zhang J et al. Cytochrome P450 2J2 inhibits the proliferation and angiogenesis of retinal vascular endothelial cells by regulating the Notch signaling pathway in a hypoxia-induced retinopathy model. Bioengineered 12:10878-10890 (2021). PubMed: 34666595
- Jin K et al. NOTCH-induced rerouting of endosomal trafficking disables regulatory T cells in vasculitis. J Clin Invest 131:N/A (2021). PubMed: 32960812