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    products/primary-antibodies/hif-1-alpha-antibody-epr16897-ab179483.pdf

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Cardiovascular Hypoxia HIF
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HIF-1 alpha antibody [EPR16897] (ab179483)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (7) Submit a question References (134)

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Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
  • Anti-HIF-1 alpha antibody [EPR16897] (ab179483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16897] to HIF-1 alpha
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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HIF-1 alpha Transcription Factor Assay Kit (ab133104)
Protein
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Recombinant Human HIF-1 alpha protein (ab154478)
Knockout
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Human HIF1A (HIF-1 alpha) knockout HCT116 cell line (ab255394)

View more associated products

Overview

  • Product name

    Anti-HIF-1 alpha antibody [EPR16897]
    See all HIF-1 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR16897] to HIF-1 alpha
  • Host species

    Rabbit
  • Specificity

    Stimulation is required for the detection of HIF-1 alpha in most samples. For better results in WB, please try 1% SDS Hot lysis method presented in applications section.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Ferret, Non human primates
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1 treated with DMOD, HeLa treated with DMOG, HeLa treated with CoCl2; NIH/3T3 treated with CoCl2, and C6 treated with CoCl2 and MG-132 (ab141003) cell lysates. ICC/IF: HeLa cells treated with DFO (1 mM, 24 h).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16897
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Hypoxia
    • HIF
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Epigenetics and Nuclear Signaling
    • Cardiovascular/Immune
    • Hypoxia
    • HIF
    • Cancer
    • Invasion/microenvironment
    • Hypoxia
    • HIF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-HIF-1 alpha antibody [EPR16897] (ab208419)
    • Alexa Fluor® 647 Anti-HIF-1 alpha antibody [EPR16897] (ab208420)
    • Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)
  • Assay kits

    • HIF-1 alpha Transcription Factor Assay Kit (ab133104)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human HIF-1 alpha protein (ab154478)
    • Recombinant Human HIF-1 alpha protein (ab48734)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • MG-132, proteasome inhibitor (ab141003)
    • Human HIF-1 alpha ELISA Kit (ab171577)
    • HIF-1 alpha (H1alpha67, EP1215Y, ESEE122, EPR16897) Antibody Panel (ab263460)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).

This antibody (ab179483) has been confirmed for Mouse sample in WB.

This antibody works better in 1% SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

ICC/IF (1)
1/500.
Notes
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).

This antibody (ab179483) has been confirmed for Mouse sample in WB.

This antibody works better in 1% SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

ICC/IF
1/500.

Target

  • Function

    Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
  • Tissue specificity

    Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Domain

    Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
  • Post-translational
    modifications

    In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
    S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
    Requires phosphorylation for DNA-binding.
    Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
    Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
  • Target information above from: UniProt accession Q16665 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281814 Cow
    • Entrez Gene: 3091 Human
    • Entrez Gene: 15251 Mouse
    • Entrez Gene: 100009579 Rabbit
    • Entrez Gene: 29560 Rat
    • Entrez Gene: 443519 Sheep
    • Omim: 603348 Human
    • SwissProt: Q16665 Human
    • SwissProt: Q61221 Mouse
    • SwissProt: O35800 Rat
    • Unigene: 597216 Human
    • Unigene: 3879 Mouse
    • Unigene: 446610 Mouse
    • Unigene: 10852 Rat
    see all
  • Alternative names

    • ARNT interacting protein antibody
    • ARNT-interacting protein antibody
    • Basic helix loop helix PAS protein MOP1 antibody
    • Basic-helix-loop-helix-PAS protein MOP1 antibody
    • bHLHe78 antibody
    • Class E basic helix-loop-helix protein 78 antibody
    • HIF 1A antibody
    • HIF 1alpha antibody
    • HIF-1-alpha antibody
    • HIF-1alpha antibody
    • HIF-alpha antibody
    • HIF1 A antibody
    • HIF1 Alpha antibody
    • HIF1 antibody
    • HIF1-alpha antibody
    • HIF1A antibody
    • HIF1A_HUMAN antibody
    • hifla antibody
    • Hypoxia inducible factor 1 alpha antibody
    • Hypoxia inducible factor 1 alpha isoform I.3 antibody
    • Hypoxia inducible factor 1 alpha subunit antibody
    • Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody
    • Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody
    • Hypoxia inducible factor1alpha antibody
    • Hypoxia-inducible factor 1-alpha antibody
    • Member of PAS protein 1 antibody
    • Member of PAS superfamily 1 antibody
    • Member of the PAS Superfamily 1 antibody
    • MOP 1 antibody
    • MOP1 antibody
    • PAS domain-containing protein 8 antibody
    • PASD 8 antibody
    • PASD8 antibody
    see all

Images

  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Wild type HAP1 treated with DMOG (0.5mM 18hr) whole cell lysate
    Lane 3 : HIF1A knockout HAP1 whole cell lysate
    Lane 4 : HIF1A knockout HAP1 treated with DMOG (0.5mM 18hr) whole cell lysate
    Lane 5 : HeLa whole cell lysate
    Lane 6 : HeLa treated with DMOG (0.5mM 18hr) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 92 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green - ab179483 observed at 105 kDa. Red - loading control, ab24834, observed at 50 kDa.

    ab179483 was shown to specifically react with HIF-1 alpha in wild-type HAP1 treated DMOG (0.5mM 18hr) cells as signal was lost in HAP1 knockout treated DMOG (0.5 mM 18hr) knockout cells. Wild-type and HAP1 knockout samples were subjected to SDS-PAGE. ab179483 and ab24834 (Mouse anti-Histone H3 loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HIF-1-alpha with ab179483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cells treated with DFO (1 mM, 24 h). The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (anti-alpha Tubulin mouse mAb) (Alexa Fluor® 594) at 1/200 dilution (red).

  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 1/5000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 0.5 mM CoCl2 for 6 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking and diluting buffer: 5% NFDM/TBST

    The different result is due to the lysates preparation method. The lysate in the left image is prepared by RIPA lysis method. The lysate in the right image is prepared by 1%SDS Hot lysis method. This antibody works better in 1%SDS Hot Lysates in WB.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 0.163 µg/ml

    Lane 1 : Untreated C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : C6 treated with 400 µM CoCl2 and 20 µM MG-132 (ab141003) for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking and diluting buffer: 5% NFDM/TBST.

    The expression of HIF-1 alpha is induced by CoCl2 and maintained by MG-132 (PMID: 15836611).

  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate treated with 0.5mM CoCl2 (Cobalt(II) chloride) for 6 hours.
    Lane 2 : Untreated HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    Western blot - Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate treated with 0.1mM CoCl2 for 24 hours.
    Lane 2 : untreated NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-HIF-1 alpha antibody [EPR16897] (ab179483)
    Anti-HIF-1 alpha antibody [EPR16897] (ab179483)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (134)

Publishing research using ab179483? Please let us know so that we can cite the reference in this datasheet.

ab179483 has been referenced in 134 publications.

  • Zhou X  et al. Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by Cobalt Chloride. World J Mens Health 41:363-372 (2023). PubMed: 35274501
  • Quelhas P  et al. HIF-1alpha-pathway activation in cholangiocytes of patients with biliary atresia: An immunohistochemical/molecular exploratory study. J Pediatr Surg 58:587-594 (2023). PubMed: 36150932
  • Xu X  et al. Chronic intermittent hypoxia accelerates cardiac dysfunction and cardiac remodeling during cardiac pressure overload in mice and can be alleviated by PHD3 overexpression. Front Cardiovasc Med 9:974345 (2022). PubMed: 36172572
  • Mohan N  et al. Effects of hypoxia on antigen presentation and T cell-based immune recognition of HPV16-transformed cells. Front Immunol 13:918528 (2022). PubMed: 36341354
  • Sun XG  et al. Aerobic Glycolysis Induced by mTOR/HIF-1α Promotes Early Brain Injury After Subarachnoid Hemorrhage via Activating M1 Microglia. Transl Stroke Res N/A:N/A (2022). PubMed: 36385451
View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HIF-1 alpha antibody [EPR16897]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Pig Tissue sections (Heart)
Antigen retrieval step
None
Permeabilization
Yes - Triton
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Iva Krumova

Verified customer

Submitted Jun 29 2022

Western blot abreview for Anti-HIF-1 alpha antibody [EPR16897]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (Stem cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
18 µg
Specification
Stem cells
Blocking step
Blotting grade blocker as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Hani Nevo

Verified customer

Submitted Feb 17 2022

Western blot abreview for Anti-HIF-1 alpha antibody [EPR16897]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (T84)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
1e+006 cells
Specification
T84
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 19 2018

Western blot abreview for Anti-HIF-1 alpha antibody [EPR16897]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Placenta)
Gel Running Conditions
Non-reduced Denaturing (4-20 %)
Loading amount
100 µg
Specification
Placenta
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 15 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-HIF-1-alpha antibody [EPR16897]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent SC derived epicardial like cells)
Specification
Human pluripotent SC derived epicardial like cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 31 2016

Western blot abreview for Anti-HIF-1-alpha antibody [EPR16897]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
2% O2
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 09 2016

Western blot abreview for Anti-HIF-1-alpha antibody [EPR16897]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle and liver)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
80 µg
Treatment
intermittent hypoxia
Specification
muscle and liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 17 2016

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