Recombinant Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
![Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)](https://www.abcam.com/ps/products/272/ab272041/Images/ab272041-464733-Anti-HIF-2-alpha-antibody-BL-95-1A2-westernblot-HepG2-HEK293T-and-786O-cells.JPG "Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha - BSA free
- Suitable for: IP, ChIP-sequencing, IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free
See all HIF-2-alpha primary antibodies -
Description
Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: IP, ChIP-sequencing, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HIF-2-alpha aa 400-450. The exact sequence is proprietary. NP_001421.2 and Gene ID 2034.
Database link: Q99814 -
Positive control
- Chip: Tumor 786-O cells. ICC/IF: HepG2 cells. IHC-P: Human renal cell carcinoma tissue. IP: HepG2 cell lysate. WB: HepG2, HEK-293T, and 786-O cells
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General notes
ab272041 is the carrier-free version of ab243861.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Carrier free
Yes -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-95-1A2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| ChIP-sequencing |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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ChIP-sequencing
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 96 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. -
Tissue specificity
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells. -
Involvement in disease
Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsIn normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
Phosphorylated on multiple sites in the CTAD.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2034 Human
- Omim: 603349 Human
- SwissProt: Q99814 Human
- Unigene: 468410 Human
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Alternative names
- Basic helix loop helix PAS protein MOP2 antibody
- Basic-helix-loop-helix-PAS protein MOP2 antibody
- bHLHe73 antibody
see all
Images
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Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)All lanes : Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041) at 1/1000 dilution
Lane 1 : HepG2 treated with 200 µM CoCl2
Lane 2 : HepG2 mock treated
Lane 3 : HEK-293T treated with 200 µM CoCl2
Lane 4 : HEK-293T mock treated
Lane 5 : 786-O cells treated with 200 µM CoCl2
Lane 6 : 786-O cells mock treated
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 96 kDaChemiluminescence with exposure time of 3 minutes.
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![ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)](https://www.abcam.com/ps/products/272/ab272041/Images/ab272041-1-ab243861334239antihif2alphaantibodybl951a2chipseq.jpg)
ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041) -
![Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)](https://www.abcam.com/ps/products/272/ab272041/Images/ab272041-2-ab243861334238antihif2alphaantibodybl951a2immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells untreated (Left) and treated with CoCl2 (right), labeling HIF-2 alpha (Red) using ab243861 at 1/100 dilution. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/100 dilution. Phalloidin Alexa Fluor® 488conjugated (green) was used as the counterstain.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).
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![Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)](https://www.abcam.com/ps/products/272/ab272041/Images/ab272041-3-ab243861334237antihif2alphaantibodybl951a2immunocytochemistry.jpg)
Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-2 alpha using ab243861 at 1/400 dilution in ICC/IF analysis. A HRP-conjugated goat-anti rabiit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)](https://www.abcam.com/ps/products/272/ab272041/Images/ab272041-4-ab243861334236antihif2alphaantibodybl951a2immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-2 alpha using ab243861 at 1/400 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).
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![Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)](https://www.abcam.com/ps/products/272/ab272041/Images/ab272041-5-ab243861334235antihif2alphaantibodybl951a2immunoprecipitation.jpg)
Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)HIF-2 alpha was immunoprecipitated from 1 mg HepG2 whole cell lysate with ab243861 at 20 µL per reaction. Western blot was performed on the immunoprecipitate using ab243861 at 1/1000 dilution.
Lane 1: ab243861 IP in HepG2 cell lysate treated with 200 µM CoCl2.
Lane 2: ab243861 IP in HepG2 whole cell lysate Mock treated.
Lane 3: Contol IgG in HepG2 cell lysate treated with 200 µM CoCl2.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).
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![Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)](https://www.abcam.com/ps/products/272/ab272041/Images/ab272041-6-benefits-of-recombinant-antibodies.png)
Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272041 has not yet been referenced specifically in any publications.