Anti-HIF1 beta antibody [2B10] (ab2771)
![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-218998-anti-hif1-beta-antibody-2b10-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)")
Key features and details
- Mouse monoclonal [2B10] to HIF1 beta
- Suitable for: Flow Cyt, WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human, Xenopus laevis, Fish, Non human primates, Zebrafish, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-HIF1 beta antibody [2B10]
See all HIF1 beta primary antibodies -
Description
Mouse monoclonal [2B10] to HIF1 beta -
Host species
Mouse -
Specificity
Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT). -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Xenopus laevis, Fish, Non human primates, Zebrafish, African green monkey
Predicted to work with: Rabbit, Cow
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Immunogen
Synthetic peptide corresponding to Human HIF1 beta aa 771-789.
Sequence:NSYNNEEFPDLTMFPPFSE
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
2B10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB | (6) |
Use at an assay dependent concentration.
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| EMSA |
Use at an assay dependent concentration.
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| Gel Shift Assay |
Use at an assay dependent concentration.
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| ICC/IF |
1/1000.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. |
|
EMSA
Use at an assay dependent concentration. |
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Gel Shift Assay
Use at an assay dependent concentration. |
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ICC/IF
1/1000. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281010 Cow
- Entrez Gene: 405 Human
- Entrez Gene: 11863 Mouse
- Entrez Gene: 100008996 Rabbit
- Entrez Gene: 25242 Rat
- Entrez Gene: 64277 Zebrafish
- Omim: 126110 Human
- SwissProt: Q9BE97 Cow
see all -
Alternative names
- arnT antibody
- ARNT protein antibody
- ARNT_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in A2058 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-218997-anti-hif1-beta-antibody-2b10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in HeLa cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-218996-anti-hif1-beta-antibody-2b10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in U251 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-6346-anti-hif1-beta-antibody-2b10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)IHC image of ab2771 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)](https://www.abcam.com/ps/products/2/ab2771/Images/ab2771-6345-anti-hif1-beta-antibody-2b10-flow-cytometry.jpg)
Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)Overlay histogram showing PC12 cells stained with ab2771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab2771 has been referenced in 21 publications.
- Ouyang L et al. miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone. iScience 25:103604 (2022). PubMed: 35005549
- Akusjärvi SS et al. Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling. iScience 25:103607 (2022). PubMed: 35005552
- Wu CS et al. ASC modulates HIF-1a stability and induces cell mobility in OSCC. Cell Death Dis 11:721 (2020). PubMed: 32883954
- Zhao Y et al. Aryl hydrocarbon receptor nuclear translocator promotes the proliferation and invasion of clear cell renal cell carcinoma cells potentially by affecting the glycolytic pathway. Oncol Lett 20:56 (2020). PubMed: 32793310
- Li C et al. microRNA-222-Mediated VHL Downregulation Facilitates Retinoblastoma Chemoresistance by Increasing HIF1a Expression. Invest Ophthalmol Vis Sci 61:9 (2020). PubMed: 32756923