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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HIF1 beta antibody [EPR17516] (ab184711)

Submit a review Submit a question References (1)
Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)
  • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
  • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
  • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
  • Anti-HIF1 beta antibody [EPR17516] (ab184711)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17516] to HIF1 beta
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HIF1 beta antibody [EPR17516]
    See all HIF1 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR17516] to HIF1 beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney, pancreas and colon lysates; wild-type HAP1 and HeLa whole cell lysates. IHC-P: Human uterus and hepatocellular carcinoma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

Target

  • Function

    Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.

  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • arnT antibody
      • ARNT protein antibody
      • ARNT_HUMAN antibody
      • Aryl hydrocarbon receptor nuclear translocator antibody
      • bHLHe2 antibody
      • Class E basic helix-loop-helix protein 2 antibody
      • Dioxin receptor antibody
      • Dioxin receptor nuclear translocator antibody
      • Drnt antibody
      • HIF 1 beta antibody
      • HIF 1beta antibody
      • HIF-1-beta antibody
      • HIF1-beta antibody
      • HIF1B antibody
      • HIF1beta antibody
      • Hypoxia Inducible Factor 1 antibody
      • Hypoxia inducible factor 1 beta antibody
      • Hypoxia-inducible factor 1-beta antibody
      • nuclear translocator antibody
      • Tango antibody
      see all

    Images

    • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      All lanes : Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : ARNT knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 87 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab184711 observed at 90 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab184711 was shown to react with ARNT in HAP1 wild-type cells in Western blot. Loss of signal was observed when ARNT knockout sample was used. HAP1 wild-type and ARNT knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab184711 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling HIF1 beta with ab184711 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on epithelial cells of Human uterus is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling HIF1 beta with ab184711 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on cancer cells of hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 87 kDa
      Observed band size: 87 kDa


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 87 kDa
      Observed band size: 87 kDa


      Exposure time: 8 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)
      All lanes : Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/2000 dilution

      Lane 1 : Human pancreas lysate
      Lane 2 : Human colon lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 87 kDa
      Observed band size: 87 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-HIF1 beta antibody [EPR17516] (ab184711)
      Anti-HIF1 beta antibody [EPR17516] (ab184711)

    References (1)

    Publishing research using ab184711? Please let us know so that we can cite the reference in this datasheet.

    ab184711 has been referenced in 1 publication.

    • Arai MA  et al. Evaluation of Naturally Occurring HIF-1 Inhibitors for Pulmonary Arterial Hypertension. Chembiochem 22:2799-2804 (2021). PubMed: 34216084

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