Recombinant Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
![Western blot - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-7-anti-hif1-beta-antibody-epr23106-55-western-blot.jpg "Western blot - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23106-55] to HIF1 beta
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-HIF1 beta antibody [EPR23106-55]
See all HIF1 beta primary antibodies -
Description
Rabbit monoclonal [EPR23106-55] to HIF1 beta -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
Unsuitable for: ChIP or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 and HeLa lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23106-55 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| WB |
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
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| IP |
1/30.
|
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| ICC/IF |
1/100.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
|
IP
1/30. |
|
ICC/IF
1/100. |
Target
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Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 405 Human
- Entrez Gene: 11863 Mouse
- Omim: 126110 Human
- SwissProt: P27540 Human
- SwissProt: P53762 Mouse
- Unigene: 632446 Human
- Unigene: 250265 Mouse
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Alternative names
- arnT antibody
- ARNT protein antibody
- ARNT_HUMAN antibody
see all
Images
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Western blot - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)All lanes : Anti-HIF1 beta antibody [EPR23106-55] (ab239366) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1:26 seconds; Lane 2:37 seconds.
Lysates should be made fresh and used in WB immediately to minimize protein degradation.
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![Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-6-anti-hif1-beta-antibody-epr23106-55-immunoprecipitation-nih-3t3-mouse.jpg)
Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)HIF1 beta was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab239366 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239366 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug
Lane 2: ab239366 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239366 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 min.
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![Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-5-anti-hif1-beta-antibody-epr23106-55-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)HIF1 beta was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab239366 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239366 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab239366 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239366 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 min.
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![Flow Cytometry (Intracellular) - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-4-anti-hif1-beta-antibody-epr23106-55-flowcytometry-nih-3t3-mouse.jpg)
Flow Cytometry (Intracellular) - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling HIF1 beta with ab239366 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-3-anti-hif1-beta-antibody-epr23106-55-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling HIF1 beta with ab239366 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-2-anti-hif1-beta-antibody-epr23106-55-immunocytochemistry-nih-3t3-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling HIF1 beta with ab239366 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear and weakly cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-1-anti-hif1-beta-antibody-epr23106-55-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling HIF1 beta with ab239366 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Anti-HIF1 beta antibody [EPR23106-55] (ab239366)](https://www.abcam.com/ps/products/239/ab239366/Images/ab239366-8-benefits-of-recombinant-antibodies.png)
Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab239366 has been referenced in 1 publication.
- Ouyang L et al. miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone. iScience 25:103604 (2022). PubMed: 35005549