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RecombinantRabMAb

Recombinant Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)

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Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
  • Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
  • Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
  • Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR27308-67] to HIF1AN/FIH-1
  • Suitable for: WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HIF1AN/FIH-1 antibody [EPR27308-67]
    See all HIF1AN/FIH-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR27308-67] to HIF1AN/FIH-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C2C12, PC-12, C6, RAW264.7 and A20 whole cell lysate. Mouse brain, heart, colon, hypothalamus and skeletal tissue lysate. Rat brain, heart and hypothalamus tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab307829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 40 kDa.
Notes
WB
1/1000. Predicted molecular weight: 40 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.

  • Sequence similarities

    Contains 1 JmjC domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp762F1811 antibody
      • Factor inhibiting HIF-1 antibody
      • Factor inhibiting HIF1 antibody
      • FIH 1 antibody
      • FIH-1 antibody
      • FIH1 antibody
      • FLJ20615 antibody
      • FLJ22027 antibody
      • HIF1AN antibody
      • HIF1N_HUMAN antibody
      • Hypoxia inducible factor 1 alpha inhibitor antibody
      • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
      • Hypoxia inducible factor asparagine hydroxylase antibody
      • Hypoxia-inducible factor 1-alpha inhibitor antibody
      • Hypoxia-inducible factor asparagine hydroxylase antibody
      • Peptide aspartate beta dioxygenase antibody
      see all

    Images

    • Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
      Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
      All lanes : Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Mouse skeletal muscle tissue lysate
      Lane 4 : Mouse hypothalamus tissue lysate
      Lane 5 : Mouse colon tissue lysate
      Lane 6 : Rat brain tissue lysate
      Lane 7 : Rat heart tissue lysate
      Lane 8 : Rat hypothalamus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 40 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 92 seconds.

    • Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
      Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
      All lanes : Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829) at 1/1000 dilution

      Lane 1 : A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate
      Lane 2 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 3 : C6 (rat glial tumor glial cell) whole cell lysate
      Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 5 : C2C12 (mouse myoblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 40 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 136 seconds.

    • Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
      Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
      All lanes : Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829) at 1/1000 dilution

      Lane 1 : C2C12 (mouse myoblast) transfected with scrambled siRNA control, whole cell lysate at 16 µg
      Lane 2 : C2C12 transfected with siRNA specifically targeting HIF1AN/FIH-1, whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 40 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.

      Exposure time: 180 seconds.

    • Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)
      Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab307829? Please let us know so that we can cite the reference in this datasheet.

    ab307829 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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