Recombinant Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)
![Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)](https://www.abcam.com/ps/products/307/ab307830/Images/ab307830-500751-anti-hif1anfih-1-antibody-epr27308-67-western-blot-mouse-brain-tissue-lysate-20-ug.jpg "Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27308-67] to HIF1AN/FIH-1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free
See all HIF1AN/FIH-1 primary antibodies -
Description
Rabbit monoclonal [EPR27308-67] to HIF1AN/FIH-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C2C12, PC-12, C6, RAW264.7 and A20 whole cell lysate. Mouse brain, heart, colon, hypothalamus and skeletal tissue lysate. Rat brain, heart and hypothalamus tissue lysate.
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General notes
ab307830 is a carrier free version of ab307829
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR27308-67 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
Target
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Function
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. -
Sequence similarities
Contains 1 JmjC domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 319594 Mouse
- Entrez Gene: 309434 Rat
- SwissProt: Q8BLR9 Mouse
- Unigene: 145278 Mouse
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Alternative names
- DKFZp762F1811 antibody
- Factor inhibiting HIF-1 antibody
- Factor inhibiting HIF1 antibody
see all
Images
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Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)All lanes : Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Mouse hypothalamus tissue lysate
Lane 5 : Mouse colon tissue lysate
Lane 6 : Rat brain tissue lysate
Lane 7 : Rat heart tissue lysate
Lane 8 : Rat hypothalamus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?This data was produced using AB307829 the same antibody clone but in a different buffer.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 92 seconds.
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![Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)](https://www.abcam.com/ps/products/307/ab307830/Images/ab307830-500750-anti-hif1anfih-1-antibody-epr27308-67-western-blot-a20-mouse-reticulum-sarcoma-b-lymphocyte-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)All lanes : Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829) at 1/1000 dilution
Lane 1 : A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate
Lane 2 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : C2C12 (mouse myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?This data was produced using AB307829 the same antibody clone but in a different buffer.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 136 seconds.
-
![Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)](https://www.abcam.com/ps/products/307/ab307830/Images/ab307830-500749-anti-hif1anfih-1-antibody-epr27308-67-western-blot-c2c12-mouse-myoblast-transfected-with-scrambled-sirna-control-whole-cell-lysate-16-ug.jpg)
Western blot - Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)All lanes : Anti-HIF1AN/FIH-1 antibody [EPR27308-67] (ab307829) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblast) transfected with scrambled siRNA control, whole cell lysate at 16 µg
Lane 2 : C2C12 transfected with siRNA specifically targeti HIF1AN/FIH-1, whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?This data was produced using AB307829 the same antibody clone but in a different buffer.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.
Exposure time: 180 seconds.
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![Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)](https://www.abcam.com/ps/products/307/ab307830/Images/ab307830-1-benefitsofrecombinantantibodies.jpg)
Anti-HIF1AN/FIH-1 antibody [EPR27308-67] - BSA and Azide free (ab307830)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab307830 has not yet been referenced specifically in any publications.
