Recombinant Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
![Western blot - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)](https://www.abcam.com/ps/products/92/ab92498/Images/ab92498-107554-anti-hif1an-fih-1-antibody-epr3658-western-blot.jpg "Western blot - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3658] to HIF1AN/FIH-1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HIF1AN/FIH-1 antibody [EPR3658]
See all HIF1AN/FIH-1 primary antibodies -
Description
Rabbit monoclonal [EPR3658] to HIF1AN/FIH-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HIF1AN/FIH-1 aa 1-100. The exact sequence is proprietary.
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Positive control
- Jurkat, 293T and A375 cell lysates; Human breast carcinoma and brain tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3658 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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WB
1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. -
Sequence similarities
Contains 1 JmjC domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Clone ID: BC007719 Human
- Entrez Gene: 55662 Human
- Omim: 606615 Human
- SwissProt: Q9NWT6 Human
- Unigene: 500788 Human
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Alternative names
- DKFZp762F1811 antibody
- Factor inhibiting HIF-1 antibody
- Factor inhibiting HIF1 antibody
see all
Images
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Western blot - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)All lanes : Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : A-375 (Human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)](https://www.abcam.com/ps/products/92/ab92498/Images/ab92498-107553-anti-hif1an-fih-1-antibody-epr3658-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)ab92498 at 1/250 dilution staining HIF1AN/FIH-1 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)](https://www.abcam.com/ps/products/92/ab92498/Images/ab92498-6-anti-hif1an-fih-1-antibody-epr3658-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HIF1AN/FIH-1 with purified ab92498 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)](https://www.abcam.com/ps/products/92/ab92498/Images/ab92498-107551-anti-hif1an-fih-1-antibody-epr3658-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)ab92498 at 1/100 dilution staining HIF1AN/FIH-1 in HeLa cells by Immunofluorescence.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)](https://www.abcam.com/ps/products/92/ab92498/Images/ab92498-107552-anti-hif1an-fih-1-antibody-epr3658-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)ab92498 at 1/250 dilution staining HIF1AN/FIH-1 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)](https://www.abcam.com/ps/products/92/ab92498/Images/ab92498-5-benefits-of-recombinant-antibodies.png)
Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab92498 has been referenced in 10 publications.
- Pei B et al. IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1a signaling pathway. Biosci Rep 40:N/A (2020). PubMed: 32347300
- Ren L et al. Influenza A virus (H1N1) triggers a hypoxic response by stabilizing hypoxia-inducible factor-1a via inhibition of proteasome. Virology 530:51-58 (2019). PubMed: 30780125
- Zhihua Y et al. Hypoxia decreases macrophage glycolysis and M1 percentage by targeting microRNA-30c and mTOR in human gastric cancer. Cancer Sci 110:2368-2377 (2019). PubMed: 31222863
- Chen Z et al. CXCL8 Promotes Glioma Progression By Activating The JAK/STAT1/HIF-1a/Snail Signaling Axis. Onco Targets Ther 12:8125-8138 (2019). PubMed: 31686858
- Yang X et al. Hypoxia-induced lncRNA EIF3J-AS1 accelerates hepatocellular carcinoma progression via targeting miR-122-5p/CTNND2 axis. Biochem Biophys Res Commun 518:239-245 (2019). PubMed: 31421822
- Ren L et al. Hypoxia-induced CCL28 promotes recruitment of regulatory T cells and tumor growth in liver cancer. Oncotarget 7:75763-75773 (2016). WB ; Human . PubMed: 27716621
- Bartoszewska S et al. The hypoxia-inducible miR-429 regulates hypoxia-inducible factor-1a expression in human endothelial cells through a negative feedback loop. FASEB J 29:1467-79 (2015). PubMed: 25550463
- Chen T et al. Factor inhibiting HIF1a (FIH-1) functions as a tumor suppressor in human colorectal cancer by repressing HIF1a pathway. Cancer Biol Ther 16:244-52 (2015). WB ; Human . PubMed: 25602156
- Wang E et al. The role of factor inhibiting HIF (FIH-1) in inhibiting HIF-1 transcriptional activity in glioblastoma multiforme. PLoS One 9:e86102 (2014). IHC ; Human . PubMed: 24465898
- Martínez-Noël G et al. Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes. Mol Cell Biol 32:3095-106 (2012). PubMed: 22645313