Anti-Histone H1 (phospho ) antibody (ab4270)
Key features and details
- Rabbit polyclonal to Histone H1 (phospho )
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Histone H1 (phospho ) antibody -
Description
Rabbit polyclonal to Histone H1 (phospho ) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Hyper-phosphorylated isoform of tetrahymena Histone H1
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.035% Sodium azide
Constituents: 1.3% Tris glycine, 30% Glycerol, 0.667% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/100.
|
|
| Flow Cyt |
Use at an assay dependent concentration. PubMed: 18250156
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/100. |
|
Flow Cyt
Use at an assay dependent concentration. PubMed: 18250156 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml). |
Target
-
Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsAcetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
-
Database links
- Entrez Gene: 3008 Human
- Entrez Gene: 50709 Mouse
- Omim: 142711 Human
- SwissProt: P10412 Human
- SwissProt: P16401 Human
- SwissProt: P43274 Mouse
- SwissProt: P43276 Mouse
- Unigene: 248133 Human
see all -
Alternative names
- H1 histone family antibody
- H14_HUMAN antibody
- H1F5 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)This image is courtesy of Dr Michael Hendzels Laboratory
Rabbit polyclonal to Histone H1 (phospho) (1/100).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).
-
Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)All lanes : Anti-Histone H1 (phospho ) antibody (ab4270) at 2 µg/ml
Lane 1 : Colcemid treated HeLa cell lysate
Lane 2 : Untreated HeLa cell lysate
Secondary
Lane 1 : Goat anti-rabbit
secondary antibody labeled with HRP
Lane 2 : Goat anti-rabbit
secondary antibody labeled with HRP.
Developed using the ECL technique.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab4270 has been referenced in 7 publications.
- Murthy T et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
- Zhang B et al. Silybin attenuates LPS-induced lung injury in mice by inhibiting NF-?B signaling and NLRP3 activation. Int J Mol Med 39:1111-1118 (2017). PubMed: 28350048
- Lei X et al. Neuroprotective effects of lycopene pretreatment on transient global cerebral ischemia-reperfusion in rats: The role of the Nrf2/HO-1 signaling pathway. Mol Med Rep 13:412-8 (2016). PubMed: 26572165
- Büchner A et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). PubMed: 25391294
- Chien WW et al. S-phase lengthening induced by p16(INK4a) overexpression in malignant cells with wild-type pRb and p53. Cell Cycle 9: (2010). WB ; Human . PubMed: 20703084
- Ingemarsdotter CK et al. Low-dose paclitaxel synergizes with oncolytic adenoviruses via mitotic slippage and apoptosis in ovarian cancer. Oncogene 29:6051-63 (2010). WB ; Human . PubMed: 20729921
- Tang X et al. A novel ATM-dependent pathway regulates protein phosphatase 1 in response to DNA damage. Mol Cell Biol 28:2559-66 (2008). Flow Cyt ; Human . PubMed: 18250156