Recombinant Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)
![Western blot - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)](https://www.abcam.com/ps/products/193/ab193203/Images/ab193203-285814-anti-histone-h2a-ubiquityl-k119-antibody-epr19800-western-blot.jpg "Western blot - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19800] to Histone H2A (ubiquityl K119)
- Suitable for: WB, ICC/IF, PepArr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Histone H2A (ubiquityl K119) antibody [EPR19800]
See all Histone H2A primary antibodies -
Description
Rabbit monoclonal [EPR19800] to Histone H2A (ubiquityl K119) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, PepArrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, NIH/3T3 and C6 whole cell lysates. ICC/IF: HeLa and NIH/3T3 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19800 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
|
|
| ICC/IF |
1/500.
|
|
| PepArr |
Use a concentration of 0.1 µg/ml.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
|
ICC/IF
1/500. |
|
PepArr
Use a concentration of 0.1 µg/ml. |
Target
-
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H2A family. -
Post-translational
modificationsThe chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
-
Database links
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8338 Human
- Entrez Gene: 319166 Mouse
- Entrez Gene: 64646 Rat
- Omim: 142720 Human
- Omim: 602786 Human
see all -
Alternative names
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
Images
-
Western blot - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)All lanes : Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : C6 (rat glial tumor cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 22 kDaExposure time : Lane 1:3 minutes; Lane 2:15 seconds; Lane 3: 3 minutes.
Blocking/Dilution buffer: 5% BSA/TBST.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here(downloadable copy).
-
![Western blot - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)](https://www.abcam.com/ps/products/193/ab193203/Images/ab193203-285812-anti-histone-h2a-ubiquityl-k119-antibody-epr19800-western-blot.jpg)
Western blot - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)All lanes : Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203) at 1/1000 dilution
All lanes : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% BSA/TBST.
The different result is due to the lysates preparation method. The lysate in lane 1 is prepared by 1%SDS Hot Lysate buffer method. The lysate in lane 2 is prepared by RIPA lysis method. This antibody works in 1%SDS Hot Lysates in WB.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
-
![Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)](https://www.abcam.com/ps/products/193/ab193203/Images/ab193203-285811-anti-histone-h2a-ubiquityl-k119-antibody-epr19800-peptide-array.jpg)
Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)ab193203 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)](https://www.abcam.com/ps/products/193/ab193203/Images/ab193203-285809-anti-histone-h2a-ubiquityl-k119-antibody-epr19800-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H2A (ubiquityl K119) with ab193203 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)](https://www.abcam.com/ps/products/193/ab193203/Images/ab193203-285808-anti-histone-h2a-ubiquityl-k119-antibody-epr19800-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Histone H2A (ubiquityl K119) with ab193203 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
![Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)](https://www.abcam.com/ps/products/193/ab193203/Images/ab193203-6-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] (ab193203)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (2)
ab193203 has been referenced in 2 publications.
- Xia YK et al. Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network. Protein Cell 12:557-577 (2021). PubMed: 32683582
- Hai DM et al. Protective Effects of Sesamin on Cytoxan-Induced Spermatogenesis Dysfunction by Regulating RNF8-ubH2A/ubH2B Pathways in Male Mice. Front Pharmacol 12:708467 (2021). PubMed: 34588981