Recombinant Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)
![Western blot - Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)](https://www.abcam.com/ps/products/214/ab214730/Images/ab214730-266526-anti-h2az-acetyl-k7-antibody-rm222-western-blot.jpg "Western blot - Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM222] to Histone H2A.Z (acetyl K7)
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-Histone H2A.Z (acetyl K7) antibody [RM222]
See all Histone H2A.Z primary antibodies -
Description
Rabbit monoclonal [RM222] to Histone H2A.Z (acetyl K7) -
Host species
Rabbit -
Specificity
ab214730 reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A. -
Tested applications
Suitable for: ELISA, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Immunogen
Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K7).
Database link: P0C0S5 -
Positive control
- Acid extracts from HeLa cells treated with sodium butyrate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM222 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 0.2 - 1 µg/ml.
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| ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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| WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.
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| Notes |
|---|
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ELISA
Use a concentration of 0.2 - 1 µg/ml. |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa. |
Target
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Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. -
Sequence similarities
Belongs to the histone H2A family. -
Post-translational
modificationsMonoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3015 Human
- Omim: 142763 Human
- SwissProt: P0C0S5 Human
- Unigene: 119192 Human
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Alternative names
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
see all
Images
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Western blot - Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)All lanes : Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730) at 0.5 µg/ml
Lane 1 : acid extracts from HeLa cells treated with sodium butyrate
Lane 2 : acid extracts from HeLa cells, untreated
Predicted band size: 14 kDa -
![ELISA - Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)](https://www.abcam.com/ps/products/214/ab214730/Images/ab214730-266528-anti-h2az-acetyl-k7-antibody-rm222-elisa.jpg)
ELISA - Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)ELISA analysis. ab214730 specifically reacts to Histone H2A.Z acetylated
at Lysine 7 (K7ac). No cross reactivity with non-modifiedLysine 7 or other acetylated Lysines in histone H2A. -
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)](https://www.abcam.com/ps/products/214/ab214730/Images/ab214730-305130-anti-h2az-acetyl-k7-antibody-rm222-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)Immunocytochemical staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate, stained for Histone H2A.Z (acetyl K7) using ab214730 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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![Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)](https://www.abcam.com/ps/products/214/ab214730/Images/ab214730-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab214730 has been referenced in 2 publications.
- Feng J et al. Characterization of Cancer Stem Cell Characteristics and Development of a Prognostic Stemness Index Cell-Related Signature in Oral Squamous Cell Carcinoma. Dis Markers 2021:1571421 (2021). PubMed: 34840626
- Wang G et al. Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica. AMB Express 7:94 (2017). WB . PubMed: 28497289