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Anti-Histone H2A.Z antibody [4A4] (ab80150)

Reviews (2)Q&A (2)
Western blot - Anti-Histone H2A.Z antibody [4A4] (ab80150)

    Key features and details

    • Rat monoclonal [4A4] to Histone H2A.Z
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG2b

    Overview

    • Product name

      Anti-Histone H2A.Z antibody [4A4]
      See all Histone H2A.Z primary antibodies

    • Description

      Rat monoclonal [4A4] to Histone H2A.Z

    • Host species

      Rat

    • Tested applications

      Suitable for: WBmore details
      Unsuitable for: Flow Cyt,ICC/IF or IHC-P

    • Species reactivity

      Reacts with: Recombinant fragment
      Predicted to work with: Mouse, Rat, Cow, Human, Pig, Xenopus laevis, Zebrafish, Orangutan

    • Immunogen

      Synthetic peptide corresponding to Human Histone H2A.Z.

    • Positive control

      • This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.

    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      IgG fraction

    • Clonality

      Monoclonal

    • Clone number

      4A4

    • Isotype

      IgG2b

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab80150 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
    Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    Target

    • Function

      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

    • Sequence similarities

      Belongs to the histone H2A family.

    • Post-translational
      modifications

      Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.

    • Cellular localization

      Nucleus. Chromosome.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    Images

    • Western blot - Anti-Histone H2A.Z antibody [4A4] (ab80150)
      Western blot - Anti-Histone H2A.Z antibody [4A4] (ab80150)
      All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/ml

      Lane 1 : Histone H2A.Z recombinant protein
      Lane 2 : Histone H2A recombinant protein

      Lysates/proteins at 0.5 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Predicted band size: 13 kDa
      Observed band size: 17 kDa why is the actual band size different from the predicted?
      Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

    References (0)

    Publishing research using ab80150? Please let us know so that we can cite the reference in this datasheet.

    ab80150 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A.Z antibody [4A4]

     Poor
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Mar 29 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A.Z antibody [4A4]

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreatic cancer)
    Specification
    Pancreatic cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    Submitted Mar 11 2010

    Question

    This is regarding the collaboration discount bellow. It looks like the antibody we were supposed to test has now been removed from the Abcam catalogue, making it difficult for us to submit an abreview…

    We have now tested the antibody in western, but are not too sure how to proceed further and how to activate our discount code.

    Thanks for your help,

    Best wishes,

    Read More
    Answer

    Thank you for contacting me.

    I am sorry that the Anti-Histone H2A.Z antibody (ab97966) has now been removed from the catalogue. I am sorry but I was not aware of this change when I suggested you test it. Let me reassure you that the antibody has not been removed due to any quality issues (if this were the case anyone who had purchased the affected antibody would have been informed).

    You should still be able to submit an Abreview for the product. This can be done by following the link below:

    https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

    And clicking on the "https://www.abcam.com/index.html?pageconfig=your_reviews&strAccessMode=public_create".

    You will need to log in to your account in order to achieve this. If you have any problems submitting the review please do let me know. As soon as the Abreview has been submitted, the discount code can be used to claim your free primary antibody (or the equivalent price of ab97966 off any product).

    I hope this information has been of help. If you have any further questions please do let me know.

    Read More

    Question


    Thanks for your help with that order. I have another question for an additional project we are initiating requiring lots of different antibodies. This is a clinical diagnostics projects, and all antibodies we will use for that need to be CE certified for diagnostic purposes. Since I did not find any information on this on your website, I wanted to ask you if you have any CE certified antibodies directed against these (human) proteins:

    Read More
    Answer

    Thank you for contacting Abcam.

    All of our products are for research purposes only and are not certified for clinical research applications.

    I am sorry that I could not be more helpful in this matter.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com