Recombinant Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)
![Peptide Array - Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)](https://www.abcam.com/ps/products/250/ab250945/Images/ab250945-3-anti-histone-h2b-phospho-t129-antibody-epr18095-bsa-and-azide-free-peptide-array.jpg "Peptide Array - Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18095] to Histone H2B (phospho T129) - BSA and Azide free
- Suitable for: PepArr, ChIP, WB
- Reacts with: Saccharomyces cerevisiae, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free
See all Histone H2B primary antibodies -
Description
Rabbit monoclonal [EPR18095] to Histone H2B (phospho T129) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: PepArr, ChIP, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250945 is the carrier-free version of ab188292.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18095 -
Isotype
IgG
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| PepArr |
Use at an assay dependent concentration.
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| ChIP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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| Notes |
|---|
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PepArr
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
Target
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Relevance
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
Cellular localization
Nuclear -
Alternative names
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
Images
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Peptide Array - Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)This data was developed using ab188292, the same antibody clone in a different buffer formulation.ab188292 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Western blot - Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)](https://www.abcam.com/ps/products/250/ab250945/Images/ab250945-403244-anti-histone-h2b-phospho-t129-antibody-epr18095-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)All lanes : Anti-Histone H2B (phospho T129) antibody [EPR18095] - ChIP Grade (ab188292) at 1/100 dilution
Lane 1 : Untreated Saccharomyces cerevisiae whole cell lysate
Lane 2 : Saccharomyces cerevisiae treated with 2 mg/ml Methyl methanesulfonate for 1 hour whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 5 secondsThis data was developed using ab188292, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![ChIP - Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)](https://www.abcam.com/ps/products/250/ab250945/Images/ab250945-1-anti-histone-h2b-phospho-t129-antibody-epr18095-bsa-and-azide-free-chip.jpg)
ChIP - Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)This data was developed using ab188292, the same antibody clone in a different buffer formulation.Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were treated with MMS at 2mg/ml for 1 h. Treated and non-treated Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188292 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). -
![Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)](https://www.abcam.com/ps/products/250/ab250945/Images/ab250945-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B (phospho T129) antibody [EPR18095] - BSA and Azide free (ab250945)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250945 has not yet been referenced specifically in any publications.
