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RecombinantRabMAb

Recombinant Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)

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ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
  • Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
  • Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
  • Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18094] to Histone H2B (yeast) - BSA and Azide free
  • Suitable for: PepArr, ChIP, WB
  • Reacts with: Saccharomyces cerevisiae, Recombinant fragment

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free
    See all Histone H2B (yeast) primary antibodies

  • Description

    Rabbit monoclonal [EPR18094] to Histone H2B (yeast) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: PepArr, ChIP, WBmore details

  • Species reactivity

    Reacts with: Saccharomyces cerevisiae, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250944 is the carrier-free version of ab188291.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18094

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
PepArr
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
Notes
PepArr
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

  • Sequence similarities

    Belongs to the histone H2B family.

  • Post-translational
    modifications

    Monoubiquitinated by the RAD6/UBC2-BRE1 complex to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and H3K79me formation. H2BK123ub1 also modulates the formation of double-strand breaks during meiosis and is a prerequisite for DNA-damage checkpoint activation. Deubiquitination is performed by UBP8 in presence of SGF11.
    Phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase. May be correlated with chromosome condensation. H2BS10ph is also formed after H(2)O(2) treatment, and is a step leading to apoptosis.
    Acetylated by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. H2BK16ac can also be formed by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
    Sumoylation to form H2BK6su or H2BK7su, and probably also H2BK16su or H2BK17su, occurs preferentially near the telomeres and represses gene transcription.

  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • H2B1_YEAST antibody
      • Histone H2B.1 antibody
      • Histone H2B.2 antibody
      • HTB 1 antibody
      • HTB 2 antibody
      • HTB1 antibody
      • Htb1p antibody
      • HTB2 antibody
      • Htb2p antibody
      • SPT 12 antibody
      • SPT12 antibody
      • Suppressor of Ty protein 12 antibody
      see all

    Images

    • ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
      ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
      This data was developed using ab188291, the same antibody clone in a different buffer formulation.Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188291 (red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    • Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
      Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
      This data was developed using ab188291, the same antibody clone in a different buffer formulation.ab188291 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

      Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

      The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
    • Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
      Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
      Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291) at 1/2000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      This data was developed using ab188291, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)
      Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250944? Please let us know so that we can cite the reference in this datasheet.

    ab250944 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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