Recombinant Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)
![ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)](https://www.abcam.com/ps/products/188/ab188291/Images/ab188291-4-AntiHistone-H2B-yeast-antibody-EPR18094--ChIP-GradeChIP.jpg "ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18094] to Histone H2B (yeast) - ChIP Grade
- Suitable for: PepArr, ChIP, WB
- Reacts with: Saccharomyces cerevisiae, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade
See all Histone H2B (yeast) primary antibodies -
Description
Rabbit monoclonal [EPR18094] to Histone H2B (yeast) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: PepArr, ChIP, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saccharomyces cerevisiae whole cell lysate. ChIP: Chromatin prepared from Saccharomyces cerevisiae cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18094 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| PepArr |
Use at an assay dependent concentration.
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| ChIP |
Use 2 µg for 25 µg of chromatin.
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| WB |
1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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| Notes |
|---|
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PepArr
Use at an assay dependent concentration. |
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ChIP
Use 2 µg for 25 µg of chromatin. |
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WB
1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H2B family. -
Post-translational
modificationsMonoubiquitinated by the RAD6/UBC2-BRE1 complex to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and H3K79me formation. H2BK123ub1 also modulates the formation of double-strand breaks during meiosis and is a prerequisite for DNA-damage checkpoint activation. Deubiquitination is performed by UBP8 in presence of SGF11.
Phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase. May be correlated with chromosome condensation. H2BS10ph is also formed after H(2)O(2) treatment, and is a step leading to apoptosis.
Acetylated by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. H2BK16ac can also be formed by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
Sumoylation to form H2BK6su or H2BK7su, and probably also H2BK16su or H2BK17su, occurs preferentially near the telomeres and represses gene transcription. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 851810 Saccharomyces cerevisiae
- Entrez Gene: 852284 Saccharomyces cerevisiae
- SwissProt: P02293 Saccharomyces cerevisiae
- SwissProt: P02294 Saccharomyces cerevisiae
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Alternative names
- H2B1_YEAST antibody
- Histone H2B.1 antibody
- Histone H2B.2 antibody
see all
Images
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ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)
Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188291 (red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
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![Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)](https://www.abcam.com/ps/products/188/ab188291/Images/ab188291-261489-anti-histone-h2b-yeast-antibody-epr18094-chip-grade-peptide-array.jpg)
Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)ab188291 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)](https://www.abcam.com/ps/products/188/ab188291/Images/ab188291-241236-anti-histone-h2b-yeast-antibody-epr18094-chip-grade-western-blot.jpg)
Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291) at 1/2000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)](https://www.abcam.com/ps/products/188/ab188291/Images/ab188291-5-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab188291 has been referenced in 5 publications.
- Yeom J & Groisman EA Reduced ATP-dependent proteolysis of functional proteins during nutrient limitation speeds the return of microbes to a growth state. Sci Signal 14:N/A (2021). PubMed: 33500334
- Jing Y et al. Semisynthesis of site-specifically succinylated histone reveals that succinylation regulates nucleosome unwrapping rate and DNA accessibility. Nucleic Acids Res 48:9538-9549 (2020). PubMed: 32766790
- Bernal M et al. The FANCM family Mph1 helicase localizes to the mitochondria and contributes to mtDNA stability. DNA Repair (Amst) 82:102684 (2019). PubMed: 31569000
- Jing Y et al. Site-Specific Installation of Succinyl Lysine Analog into Histones Reveals the Effect of H2BK34 Succinylation on Nucleosome Dynamics. Cell Chem Biol 25:166-174.e7 (2018). PubMed: 29249693
- Shen D & Skibbens RV Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition. PLoS One 12:e0188739 (2017). ChIP . PubMed: 29186203