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    products/primary-antibodies/histone-h3-acetyl-k27-antibody-chip-grade-ab4729.pdf

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Epigenetics and Nuclear Signaling Histones H3 Acetylated
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Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

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  • SDS
Reviews (82)Q&A (8)References (1539)

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ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

Key features and details

  • Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade
  • Suitable for: ICC/IF, WB, IHC-P, ChIP, PepArr
  • Reacts with: Mouse, Rat, Cow, Human, Recombinant fragment
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Histone H3 (acetyl K27) antibody - ChIP Grade
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ChIP, PepArrmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Recombinant fragment
    Predicted to work with: Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Plasmodium falciparum, Rice, Cyanidioschyzon merolae
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (acetyl K27) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24404)

  • Positive control

    • WB : HeLa (human cervix adenocarcinoma epithelial cell) cell lysate - Sodium butyrate-treated, HeLa (human cervix adenocarcinoma epithelial cell) nuclear lysate (triton enriched), NIH/3T3 (mouse embryonic fibroblast cell line) nuclear lysate (triton enriched) and PC-12 (rat adrenal gland pheochromocytoma cell) nuclear lysate (triton enriched).
  • General notes

    Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Signal Transduction
    • Antibodies
    • histone h3

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Control Peptide

    • Human Histone H3 (acetyl K9) peptide (ab16635)
    • Human Histone H3 (acetyl K23) peptide (ab48359)
  • Immunizing Peptide (Blocking)

    • Human Histone H3 (acetyl K27) peptide (ab24404)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • GAPDH positive control ChIP primer pair (ab267832)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Human Histone H3 (tri methyl K4) peptide (ab1342)
    • Human Histone H3 (tri methyl K9) peptide (ab1773)
    • Human Histone H3 (tri methyl K27) peptide (ab1782)
    • Human Histone H3 (unmodified) peptide (ab7228)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (22)
Use a concentration of 0.5 µg/ml.

Can be used with paraformaldehyde- or methanol- fixed cells.

WB (20)
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

IHC-P (4)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP (27)
Use 2 µg for 25 µg of chromatin.

We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control.

PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
Notes
ICC/IF
Use a concentration of 0.5 µg/ml.

Can be used with paraformaldehyde- or methanol- fixed cells.

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP
Use 2 µg for 25 µg of chromatin.

We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control.

PepArr
Use a concentration of 0.2 - 0.02 µg/ml.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H3 family.
  • Developmental stage

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P84229 Chicken
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • Alternative names

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

Images

  • ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4729 (blue), and 20 µl of Protein A/G sepharose beads.

    No antibody was added to the beads control (yellow).

    The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    ab4729 staining Histone H3 (acetyl K27) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were incubated with 10 mM sodium butyrate (ab120948) for 6 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab4729 at 0.5 µg/ml and ab7291 at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with a anti-rabbit AlexaFluor®488 secondary antibody (ab150077) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in pseudo colour red). Nuclear DNA was labeled in blue with DAPI.

    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    IHC image of ab4729 staining Histone H3 (acetyl K27) in human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6 for 20 minutes. The section was then incubated with ab4729, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    All batches of ab4729 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K27 peptide (ab24404), indicating that this antibody specifically recognises the Histone H3 - acetyl K27 modification.

    ab24404 - Histone H3 - acetyl K27

    ab15591 - Histone H3 - acetyl K14

    ab24003 - Histone H3 - acetyl K18

    ab17163 - Histone H3 unmodified

    ab48359 - Histone H3 - acetyl K23

    ab41409 - Histone H3 - acetyl K36

    ab15662 - Histone H4 - acetyl K12

    ab16635 - Histone H3 acetyl K9

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    All lanes : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 1 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) cell lysate - Sodium butyrate-treated
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) nuclear lysate (triton enriched)
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast cell line) nuclear lysate (triton enriched)
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) nuclear lysate (triton enriched)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking buffer : 2% BSA block

    Gel type : MES

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    All lanes : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 1/2500 dilution

    Lane 1 : Untreated Mouse MEF cell lysate
    Lane 2 : 0.4 µM Trichostatin A treatment for 18 hr Mouse MEF cell lysate

    Lysates/proteins at 9 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution

    Predicted band size: 15 kDa

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)This image is courtesy of Petra Hajkova - Gurdon Institute, Cambridge University

    Primary antibody: ab4729 (H3 acetyl K27)
    Dilution: 1/100

    ab4729 strongly stained histones of mouse ES cells. However, fluroescence was greatly diminished following pre-blocking using a H3 acetyl K9 peptide. This suggests the antibody cross-reacts with the K9 and K27 residues.

     

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Lanes 1 & 3 : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 0.2 µg/ml
    Lanes 2 & 4 : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 0.1 µg/ml

    Lanes 1-2 : Calf thymus histone lysate
    Lanes 3-4 : Calf thymus histone lysate with Human Histone H3 (acetyl K27) peptide (ab24404) at 2 µg

    Lysates/proteins at 1 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit (HRP) at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    ab4729 specifically recognises acetyl K27 histone H3 in catlf thymus histone lysate, which is specifically blocked using the immunizing peptide ab24404.
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were incubated at 37°C for 6 hours with vehicle control (0 μM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27)(ab4729) in HeLa cells correlates with an increase in sodium butyrate concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 2.5 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4927 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10,000 dilution and visualised using ECL development solution.

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation, nuclear Lysate - Butyrate treated at 2.5 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds


     

     

Protocols

  • PCR primers used to test ab4729 in ChIP at Abcam

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1539)

Publishing research using ab4729? Please let us know so that we can cite the reference in this datasheet.

ab4729 has been referenced in 1539 publications.

  • Chen Z  et al. Imaging assisted evaluation of antitumor efficacy of a new histone deacetylase inhibitor in the castration-resistant prostate cancer. Eur J Nucl Med Mol Imaging 48:53-66 (2021). PubMed: 32592040
  • Wei J  et al. Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell 184:76-91.e13 (2021). PubMed: 33147444
  • Caputo T  et al. Anti-adipogenic signals at the onset of obesity-related inflammation in white adipose tissue. Cell Mol Life Sci 78:227-247 (2021). PubMed: 32157317
  • Qi X  et al. Brg1 restrains the pro-inflammatory properties of ILC3s and modulates intestinal immunity. Mucosal Immunol 14:38-52 (2021). PubMed: 32612160
  • Theisen ER  et al. Chromatin profiling reveals relocalization of lysine-specific demethylase 1 by an oncogenic fusion protein. Epigenetics 16:405-424 (2021). PubMed: 32842875
View all Publications for this product

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
Yes - 0.2% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Nov 10 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Embryonic stem cell)
Permeabilization
Yes - 0.5% Triton
Specification
Embryonic stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 01 2022

Immunohistochemistry (Frozen sections) abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (fetal germline)
Permeabilization
No
Specification
fetal germline
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 01 2022

Western blot abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Smooth Muscle cells)
Gel Running Conditions
Reduced Denaturing (4-15)
Loading amount
15 µg
Specification
Smooth Muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 15 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Colorectal carcinoma)
Permeabilization
Yes - Tween
Specification
Colorectal carcinoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 13 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer at 75C
Permeabilization
Yes - 3% Triton-X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Madeline Clark

Verified customer

Submitted Oct 28 2021

Western blot abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
Mouse vascular smooth muscle cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 25 2021

Western blot abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
Human vascular smooth muscle cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 25 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 25 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Human vascular smooth muscle cells)
Permeabilization
Yes - NP40
Specification
Human vascular smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 25 2021

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