Recombinant Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (ab300641)
![Dot Blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (ab300641)](https://www.abcam.com/ps/products/300/ab300641/Images/ab300641-498712-anti-histone-h3acetyl-k4k9k14k18k23k27-antibody-oligo45-dot-blot-histone-h3.jpg "Dot Blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (ab300641)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1045] to Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27)
- Suitable for: IHC-P, PepArr, Flow Cyt (Intra), ICC, WB, IHC-Fr, IP, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045]
See all Histone H3 primary antibodies -
Description
Rabbit recombinant multiclonal [RM1045] to Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, PepArr, Flow Cyt (Intra), ICC, WB, IHC-Fr, IP, Dot blotmore details
Unsuitable for: ChIP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- WB: HeLa, NIH/3T3, and C6 treated with TSA, whole cell lysates. Dot Blot:Histone H3 modified and unmodified peptide. IHC-P:Human, mouse, and rat colon; human Prostatic hyperplasia, FFPE tissue sections. IHC-Fr:Mouse and rat colon fresh frozen tissues. ICC: HeLa and NIH/3T3 cells Flow Cyt (Intra): HeLa and NIH/3T3 treated with TSA IP: HeLa and NIH/3T3 treated with TSA PA: modified and unmodified histone peptides.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Recombinant Multiclonal -
Clone number
RM1045 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| PepArr |
Use a concentration of 0.1 µg/ml.
|
|
| Flow Cyt (Intra) |
1/500.
|
|
| ICC |
1/50.
|
|
| WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
|
|
| IHC-Fr |
1/500.
|
|
| IP |
1/30.
|
|
| Dot blot |
1/1000.
|
| Notes |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
PepArr
Use a concentration of 0.1 µg/ml. |
|
Flow Cyt (Intra)
1/500. |
|
ICC
1/50. |
|
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). |
|
IHC-Fr
1/500. |
|
IP
1/30. |
|
Dot blot
1/1000. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H3 family. -
Developmental stage
Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. -
Post-translational
modificationsAcetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 8350 Human
- Entrez Gene: 8351 Human
- Entrez Gene: 8352 Human
- Entrez Gene: 8353 Human
- Entrez Gene: 8354 Human
- Entrez Gene: 8355 Human
- Entrez Gene: 8356 Human
- Entrez Gene: 8357 Human
see all -
Alternative names
- H3 histone family member E pseudogene antibody
- H3 histone family, member A antibody
- H3/A antibody
see all
Images
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Dot Blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (ab300641)
Dot blot analysis of Histone H3(acetyl K4+K9+K14+K18+K23+K27) using AB300641 at 1: 1000 dilution (0.477 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:10,000 dilution.
Lane 1: Histone H3 (acetyl K4) peptide
Lane 2: Histone H3 (acetyl K9) peptide
Lane 3: Histone H3 (acetyl K14) peptide
Lane 4: Histone H3 (acetyl K18) peptide
Lane 5: Histone H3 (acetyl K23) peptide
Lane 6: Histone H3 (acetyl K27) peptide
Lane 7: Histone H3 unmodified peptideExposure time: 40 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST -
![Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-488252-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-rm1045-western-blot-3-c6-rat-glial-tumor-cell-lysate.jpg)
Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)All lanes : Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (ab300641) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 treated with 500ng/ml TSA for 4h whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocking / Diluting buffer and concentration: 5% NFDM/TBST
Similar doublet band pattern in rat species and single band in human and mouse species was also observed with benchmark and other company's product.
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![Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-488251-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-rm1045-western-blot-1-nih-3t3.jpg)
Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)All lanes : Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (ab300641) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 treated with 500ng/ml TSA for 4h whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocking / Diluting buffer and concentration: 5% NFDM/TBST
-
![Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-488248-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-rm1045-western-blot-1-hela.jpg)
Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)All lanes : Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (ab300641) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 500ng/ml TSA for 4h whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocking / Diluting buffer and concentration: 5% NFDM/TBST
-
![Peptide Array - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-13-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-antibody-oligo45-peptide-array-histone-peptides.jpg)
Peptide Array - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Peptide array analysis of ab300641 at 0.1µg/ml followed by a Goat Anti-Rabbit IgG, (H+L), (647nm) conjµgate at 1:50,000 dilution.
All batches of ab300641 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. Scanner details, Innoscan 710.
The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Immunoprecipitation - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-12-anti-histone-h3-acetyl-k4-k9--k14--k18--k23--k27-antibody-oligo45-immunoprecipitation-nih3t3.jpg)
Immunoprecipitation - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Histone H3(acetyl K4+K9+K14+K18+K23+K27) was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) treated with 500ng/ml TSA for 4h whole cell lysate 10 µg with ab300641 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300641 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 (Input): NIH/3T3 (mouse embryonic fibroblast) treated with 500ng/ml TSA for 4h whole cell lysate 10 µg
Lane 2 (+): ab300641 IP in NIH/3T3 treated with 500ng/ml TSA for 4h whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab300641 in NIH/3T3 treated with 500ng/ml TSA for 4h whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 100 seconds
-
![Immunoprecipitation - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-11-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-antibody-oligo45-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Histone H3(acetyl K4+K9+K14+K18+K23+K27) was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) treated with 500ng/ml TSA for 4h whole cell lysate 10 µg with ab300641 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300641 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 (Input): HeLa (human cervical adenocarcinoma epithelial cell) treated with 500ng/ml TSA for 4h whole cell lysate 10 µg
Lane 2 (+): ab300641 IP in HeLa treated with 500ng/ml TSA for 4h whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab300641 in HeLa treated with 500ng/ml TSA for 4h whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 100 seconds
-
![Immunohistochemistry (Frozen sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-10-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-antibody-oligo45-immunohistochemistry-frozen-rat-colon.jpg)
Immunohistochemistry (Frozen sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Immunohistochemical analysis of 4% PFA-fixed/ 0.2% Triton X-100 permeabilized frozen rat colon (fresh) tissue labeling Histone H3(acetyl K4 + K9 + K14 + K18 + K23 + K27) with ab300641 at 1/500 dilution (0.954 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml) (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
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![Immunohistochemistry (Frozen sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-9-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-antibody-oligo45-immunohistochemistry-frozen-mouse-colon.jpg)
Immunohistochemistry (Frozen sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Immunohistochemical analysis of 4% PFA-fixed / 0.2% Triton X-100 permeabilized frozen mouse colon (fresh) tissue labeling Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27) with ab300641 at 1/500 (0.954 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml) (Green). Positive staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
-
![Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-8-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-antibody-oligo45-intracellular-flow-cytometry-nih3t3.jpg)
Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblasts) treated with 500ng/ml TSA for 4h (Red) / Untreated control (Green) labeling Histone H3 (acetyl K4+K9+K14+K18+K23+K27) with ab300641 at 1/500 dilution (0.1µg) Red and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-7-anti-histone-h3-acetyl-k4--k9--k14--k18--k23--k27-antibody-oligo45-intracellular-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cells) treated with 500ng/ml TSA for 4h (Red) / Untreated control (Green) cells labeling Histone H3 (acetyl K4+K9+K14+K18+K23+K27) with ab300641 at 1/500 dilution (0.1µg) Red and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-6-anti-histone-h3-acetyl-k4-k9-k14--k18--k23--k27-antibody-oligo45-immunohistochemistry-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Immunohistochemical analysis of paraffin-embedded rat colon tissue labelling Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27) with ab300641 at 1/2000 dilution (0.239 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Nuclear staining on rat colon. The section was incubated with ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with citrate buffer (pH 6.0 epitope retrieval solution 1) for 20 mins was used
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-5-anti-histone-h3-acetyl-k4-k9--k14--k18--k23--k27-antibody-oligo45-immunohistochemistry-mouse-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27) with ab300641 at 1/2000 dilution (0.239 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Nuclear staining on mouse colon. The section was incubated with ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with citrate buffer (pH 6.0 epitope retrieval solution 1) for 20 mins was used
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-4-anti-histone-h3-acetyl-k4k9k14k18k23k27-antibody-oligo45-immunohistochemistry-human-prostatic-hyperplasia.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labelling Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27) with ab300641 at 1/2000 dilution (0.239 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Nuclear staining on human prostatic hyperplasia. The section was incubated with ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with citrate buffer (pH 6.0 epitope retrieval solution 1) for 20 mins was used
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-3-anti-histone-h3-acetyl-k4k9k14k18k23k27-antibody-oligo45-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27) with ab300641 at 1/2000 dilution (0.239 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human tonsil was observed. The section was incubated with ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with citrate buffer (pH 6.0 epitope retrieval solution 1) for 20 mins was used
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-2-anti-histone-h3-acetyl-k4k9k14k18k23k27-antibody-oligo45-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H3 (acetyl K4 + K9 + K14 + K18 + K23 + K27) with ab300641 at 1/2000 dilution (0.239 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Nuclear staining on human colon was observed. The section was incubated with ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with citrate buffer (pH 6.0 epitope retrieval solution 1) for 20 mins was used
-
![Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)](https://www.abcam.com/ps/products/300/ab300641/Images/AB300641-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (AB300641)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300641 has not yet been referenced specifically in any publications.