Name: Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade
Brand: RabMAb
SKU: ab32129
Rating: 5 (6 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y28] to Histone H3 (acetyl K9) - ChIP Grade
Suitable for: Flow Cyt (Intra), ChIP-sequencing, ChIP, WB, IHC-P, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Product name

Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [Y28] to Histone H3 (acetyl K9) - ChIP Grade
Host species

Rabbit
Specificity

This antibody may be cross reactive with other acetyl sites such as k4, k14 and k18.
Tested applications

Suitable for: Flow Cyt (Intra), ChIP-sequencing, ChIP, WB, IHC-P, IP, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Histone H3 aa 1-100. The exact sequence is proprietary.
Database link: P68431
Positive control
- WB: HeLa (Human cervix adenocarcinoma epithelial cell), NIH/3T3 (Mouse embryonic fibroblast) and C6 (Rat glial tumor glial cell) treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate IHC-P: Human cerebrum, colorectal carcinoma, mouse colon and rat colon tissue sections. ChIP: Chromatin prepared from HeLa cells. ChIP-seq: Chromatin prepared from HeLa cells. IP: HeLa cells. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y28
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human Histone H3 protein (Active) (ab198757)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		Use at an assay dependent concentration.
ChIP-sequencing		Use 4 µg for 30 µg of chromatin.
ChIP		Use 2 µg for 25 µg of chromatin.
WB	(4)	1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
IHC-P	(1)	1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP		1/30.
ICC/IF	(1)	1/250.

Notes
Flow Cyt (Intra) Use at an assay dependent concentration.
ChIP-sequencing Use 4 µg for 30 µg of chromatin.
ChIP Use 2 µg for 25 µg of chromatin.
WB 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/30.
ICC/IF 1/250.

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similarities

Belongs to the histone H3 family.
Developmental stage

Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
Post-translational
modifications

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
Cellular localization

Nucleus. Chromosome.
Target information above from: UniProt accession P68431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 8350 Human
- Entrez Gene: 8351 Human
- Entrez Gene: 8352 Human
- Entrez Gene: 8353 Human
- Entrez Gene: 8354 Human
- Entrez Gene: 8355 Human
- Entrez Gene: 8356 Human
- Entrez Gene: 8357 Human
- Entrez Gene: 8358 Human
- Entrez Gene: 8968 Human
- Entrez Gene: 319152 Mouse
- Entrez Gene: 319153 Mouse
- Entrez Gene: 360198 Mouse
- Entrez Gene: 97908 Mouse
- Entrez Gene: 100364501 Rat
- Entrez Gene: 100365669 Rat
- Entrez Gene: 291159 Rat
- Entrez Gene: 679994 Rat
- Entrez Gene: 680511 Rat
- Entrez Gene: 682330 Rat
- Omim: 602810 Human
- SwissProt: P68431 Human
- SwissProt: P68433 Mouse
- SwissProt: Q6LED0 Rat
- Unigene: 132854 Human
- Unigene: 247813 Human
- Unigene: 247814 Human
- Unigene: 248176 Human
- Unigene: 443021 Human
- Unigene: 484990 Human
- Unigene: 532144 Human
- Unigene: 533292 Human
- Unigene: 546315 Human
- Unigene: 586261 Human
- Unigene: 591778 Human
- Unigene: 221301 Mouse
- Unigene: 261657 Mouse
- Unigene: 377874 Mouse
- Unigene: 390558 Mouse
- Unigene: 397328 Mouse
- Unigene: 138090 Rat
see all
Alternative names
- H3 histone family member E pseudogene antibody
- H3 histone family, member A antibody
- H3/A antibody
- H31_HUMAN antibody
- H3F3 antibody
- H3FA antibody
- Hist1h3a antibody
- HIST1H3B antibody
- HIST1H3C antibody
- HIST1H3D antibody
- HIST1H3E antibody
- HIST1H3F antibody
- HIST1H3G antibody
- HIST1H3H antibody
- HIST1H3I antibody
- HIST1H3J antibody
- HIST3H3 antibody
- histone 1, H3a antibody
- Histone cluster 1, H3a antibody
- Histone H3 3 pseudogene antibody
- Histone H3.1 antibody
- Histone H3/a antibody
- Histone H3/b antibody
- Histone H3/c antibody
- Histone H3/d antibody
- Histone H3/f antibody
- Histone H3/h antibody
- Histone H3/i antibody
- Histone H3/j antibody
- Histone H3/k antibody
- Histone H3/l antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Immunohistochemical analysis of Paraffin-embedded sections rat colon tissue labelling Histone H3 (acetyl K9) with ab32129 at 1/6000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat colon tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Immunohistochemical analysis of Paraffin-embedded sections mouse colon tissue labelling Histone H3 (acetyl K9) with ab32129 at 1/6000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse colon tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Immunohistochemical analysis of Paraffin-embedded sections human colorectal carcinoma tissue labelling Histone H3 (acetyl K9) with ab32129 at 1/2000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human colorectal carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Immunohistochemical analysis of Paraffin-embedded sections human cerebrum tissue labelling Histone H3 (acetyl K9) with ab32129 at 1/2000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human cerebrum tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Western blot - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

All lanes : Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129) at 1/10000 dilution

Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

ab181602 was used as GAPDH loading control.

ab176842 was for Histone H3 detection.
Western blot - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

All lanes : Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129) at 1/10000 dilution

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

ab181602 was used as GAPDH loading control.

ab176842 was for Histone H3 detection.
Western blot - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

All lanes : Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129) at 1/10000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

ab181602 was used as GAPDH loading control.

ab176842 was for Histone H3 detection.
ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ HeLa cells and 4 µg of Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129). ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

Additional screenshots of mapped reads can be downloaded here.
ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129). ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed by Active Motif, Carlsbad, CA.

Additional screenshots of mapped reads can be downloaded here.
ChIP - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 2 µg of ab32129 (red), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
Primers and probes are located in the first kb of the transcribed region.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Histone H3 with purified ab32129 at 1/250 dilution (4 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
Immunoprecipitation - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

ab32129 (purified) at 1/30 dilution (20 µg/ml) immunoprecipitating Histone H3 acetyl K9 in TSA treated HeLa whole cell lysate.
Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) treated with 500ng/ml TSA for 4h whole cell lysate 10µg
Lane 2 (+): ab32129 & TSA treated HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32129 in TSA treated HeLa whole cell lysate
For western blotting, ab32129 at 1/500 and veriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST.
Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab32129 at 1/230 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

Download

Publishing research using ab32129? Please let us know so that we can cite the reference in this datasheet.

ab32129 has been referenced in 38 publications.

Cui X et al. HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility. Hepatol Int 17:377-392 (2023). PubMed: 36508088
Han B et al. Epigenetic regulation of seed-specific gene expression by DNA methylation valleys in castor bean. BMC Biol 20:57 (2022). PubMed: 35227267
Zhang Q et al. Targeting a cryptic allosteric site of SIRT6 with small-molecule inhibitors that inhibit the migration of pancreatic cancer cells. Acta Pharm Sin B 12:876-889 (2022). PubMed: 35256952
Sanders JT et al. Loops, topologically associating domains, compartments, and territories are elastic and robust to dramatic nuclear volume swelling. Sci Rep 12:4721 (2022). PubMed: 35304523
Ji L et al. Ketone Body β-Hydroxybutyrate Prevents Myocardial Oxidative Stress in Septic Cardiomyopathy. Oxid Med Cell Longev 2022:2513837 (2022). PubMed: 35340211

View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application

Immunocytochemistry/ Immunofluorescence

Sample

Daphnia magna Cell (Embryonic cells)

Permeabilization

Specification

Embryonic cells

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 21 2023

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate (pH 6)

Permeabilization

Yes - PBS-Tween

Specification

Colon

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 11 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (T-cell acute lymphoblastic leukemia cells)

Gel Running Conditions

Reduced Denaturing (12)

Loading amount

45 µg

Specification

T-cell acute lymphoblastic leukemia cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (T-cell acute lymphoblastic leukemia cells)

Gel Running Conditions

Reduced Denaturing (12)

Loading amount

45 µg

Specification

T-cell acute lymphoblastic leukemia cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (Cell line)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

90 µg

Specification

Cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Progenitor T cell line)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

45 µg

Specification

Progenitor T cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2022

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Developmental stage

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (38)

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade

Western blot abreview for Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade

Western blot abreview for Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade

Western blot abreview for Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade

Western blot abreview for Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade

Post-translational
modifications