Anti-Histone H3 (phospho S10) antibody (ab183626)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho S10)
- Suitable for: ChIP, IP, IHC - Wholemount, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
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Overview
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Product name
Anti-Histone H3 (phospho S10) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho S10) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, IP, IHC - Wholemount, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Chimpanzee
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Immunogen
Synthetic peptide within Human Histone H3 (phospho S10). The exact sequence is proprietary. Synthetic phospho-peptide corresponding to amino acids residues surrounding the phospho Ser10.
Database link: P68431 -
Positive control
- U2OS (100ng/ml Nocodazole treatment for 24hr) , 4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr); RFP-tag transfected HeLa cells; Mitotic HeLa cells; Human gastric carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
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| IP |
1/100 - 1/500.
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| IHC - Wholemount |
1/100 - 1/500.
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| IHC-P |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 15 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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ChIP
Use at an assay dependent concentration. |
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IP
1/100 - 1/500. |
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IHC - Wholemount
1/100 - 1/500. |
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IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 15 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H3 family. -
Developmental stage
Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. -
Post-translational
modificationsAcetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 8350 Human
- Entrez Gene: 8351 Human
- Entrez Gene: 8352 Human
- Entrez Gene: 8353 Human
- Entrez Gene: 8354 Human
- Entrez Gene: 8355 Human
- Entrez Gene: 8356 Human
- Entrez Gene: 8357 Human
see all -
Alternative names
- H3 histone family member E pseudogene antibody
- H3 histone family, member A antibody
- H3/A antibody
see all
Images
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ChIP - Anti-Histone H3 (phospho S10) antibody (ab183626)
Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3 (phospho S10) antibody (ab183626). The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.
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Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)All lanes : Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution
Lane 1 : U2OS whole cell lysate (untreated)
Lane 2 : U2OS whole cell lysate (serum deprivation for 48hr)
Lane 3 : U2OS whole cell lysate (100ng/ml Nocodazole treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (ab183626)Confocal immunofluorescent analysis of mitotic HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red: Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/2500 . Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Histone H3 (phospho S10) with ab183626 at 1/500.
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Immunoprecipitation - Anti-Histone H3 (phospho S10) antibody (ab183626)ab183626 immunoprecipitating Histone H3 in HeLa whole cell lysate.
Lane (-): Untreated HeLa whole cell lysate (30 µg)
Lane (+): Treated HeLa whole cell lysate (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ab183626 diluted at 1:2000.
For western blotting, HRP-conjugated anti-rabbit IgG was used as a secondary antibody.
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Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)All lanes : Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/500 dilution
Lane 1 : Rat2 whole cell lysate
Lane 2 : Rat2 whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunofluorescent analysis of RFP-tag transfected HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red: Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/500 . Blue: Hoechst 33342 staining.
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Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)All lanes : Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution
Lane 1 : 4T1 whole cell lysate
Lane 2 : 4T1 whole cell lysate (100ng/ml Nocodazole treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa -
IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
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IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183626 has not yet been referenced specifically in any publications.