Recombinant Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)
![Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-232071-anti-histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-western-blot.jpg "Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16606] to Histone H4 (acetyl K5 + K8 + K12 + K16)
- Suitable for: PepArr, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [EPR16606] to Histone H4 (acetyl K5 + K8 + K12 + K16) -
Host species
Rabbit -
Tested applications
Suitable for: PepArr, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa treated with Sodium butyrate extract lysates; NIH/3T3 treated with Trichostatin A whole cell lysate. IHC-P: Human, Mouse and Rat colon tissues. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16606 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| PepArr |
Use at an assay dependent concentration.
|
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| WB | (1) |
1/20000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
|
| IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| ICC/IF |
1/5000.
|
| Notes |
|---|
|
PepArr
Use at an assay dependent concentration. |
|
WB
1/20000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/5000. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)All lanes : Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790) at 1/20000 dilution
Lane 1 : HeLa treated with Sodium butyrate extract lysates
Lane 2 : Untreated HeLa extract lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-251610-anti-histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/5000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The expression increased after treatment with TSA (50 μg/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab177790 at 1/15000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-232067-anti-histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-232069-anti-histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-western-blot.jpg)
Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)All lanes : Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790) at 1/100000 dilution
Lane 1 : NIH/3T3 treated with Trichostatin A whole cell lysates
Lane 2 : Untreated NIH/3T3 whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-232068-anti-histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-peptide-array.jpg)
Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)ab177790 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-232066-anti-histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of mouse colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-232063-anti-histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of rat colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)](https://www.abcam.com/ps/products/177/ab177790/Images/ab177790-10-benefits-of-recombinant-antibodies.png)
Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (23)
ab177790 has been referenced in 23 publications.
- Yue Y et al. Topography of histone H3-H4 interaction with the Hat1-Hat2 acetyltransferase complex. Genes Dev 36:408-413 (2022). PubMed: 35393344
- Sinniah E et al. Temporal perturbation of histone deacetylase activity reveals a requirement for HDAC1-3 in mesendoderm cell differentiation. Cell Rep 39:110818 (2022). PubMed: 35584683
- Wang R et al. The HN1/HMGB1 axis promotes the proliferation and metastasis of hepatocellular carcinoma and attenuates the chemosensitivity to oxaliplatin. FEBS J 289:6400-6419 (2022). PubMed: 35596723
- Liu H et al. Osteocyte CIITA aggravates osteolytic bone lesions in myeloma. Nat Commun 13:3684 (2022). PubMed: 35760800
- Bühler A et al. Histone deacetylase 1 controls cardiomyocyte proliferation during embryonic heart development and cardiac regeneration in zebrafish. PLoS Genet 17:e1009890 (2021). PubMed: 34723970