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    products/primary-antibodies/histone-h4-antibody-epr16599-chip-grade-ab177840.pdf

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RecombinantRabMAb

Recombinant Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (11)

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ChIP - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Peptide Array - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16599] to Histone H4 - ChIP Grade
  • Suitable for: PepArr, ChIP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Recombinant fragment

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Histone H4 antibody [EPR16599] - ChIP Grade
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [EPR16599] to Histone H4 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: PepArr, ChIP, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Recombinant fragment
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and NIH/3T3 whole cell lysates; Drosophila whole lysate. ICC/IF: HeLa cells. IHC-P: Human colon, mouse pancreas and rat cerebral cortex tissues. ChIP: Chromatin from HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16599
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Histone H4 antibody [EPR16599] (ab207075)
    • Alexa Fluor® 488 Anti-Histone H4 antibody [EPR16599] (ab207387)
    • HRP Anti-Histone H4 antibody [EPR16599] (ab208870)
    • Anti-Histone H4 antibody [EPR16599] - BSA and Azide free (ab232371)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)
  • Related Products

    • Histone H4 Total Quantification Kit (Colorimetric) (ab156909)
    • Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
PepArr
Use at an assay dependent concentration.
ChIP
Use 2 µg for 25 µg of chromatin.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (4)
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

ICC/IF
1/100.
Notes
PepArr
Use at an assay dependent concentration.
ChIP
Use 2 µg for 25 µg of chromatin.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

ICC/IF
1/100.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 318846 Drosophila melanogaster
    • Entrez Gene: 3771854 Drosophila melanogaster
    • Entrez Gene: 3771893 Drosophila melanogaster
    • Entrez Gene: 3771908 Drosophila melanogaster
    • Entrez Gene: 3771935 Drosophila melanogaster
    • Entrez Gene: 3771938 Drosophila melanogaster
    • Entrez Gene: 3771941 Drosophila melanogaster
    • Entrez Gene: 3771947 Drosophila melanogaster
    • Entrez Gene: 3772096 Drosophila melanogaster
    • Entrez Gene: 3772113 Drosophila melanogaster
    • Entrez Gene: 3772129 Drosophila melanogaster
    • Entrez Gene: 3772172 Drosophila melanogaster
    • Entrez Gene: 3772211 Drosophila melanogaster
    • Entrez Gene: 3772254 Drosophila melanogaster
    • Entrez Gene: 3772314 Drosophila melanogaster
    • Entrez Gene: 3772317 Drosophila melanogaster
    • Entrez Gene: 3772319 Drosophila melanogaster
    • Entrez Gene: 3772325 Drosophila melanogaster
    • Entrez Gene: 3772509 Drosophila melanogaster
    • Entrez Gene: 3772519 Drosophila melanogaster
    • Entrez Gene: 3772666 Drosophila melanogaster
    • Entrez Gene: 3772708 Drosophila melanogaster
    • Entrez Gene: 41773 Drosophila melanogaster
    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 64627 Rat
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P84040 Drosophila melanogaster
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P62804 Rat
    • Unigene: 21500 Drosophila melanogaster
    • Unigene: 29514 Drosophila melanogaster
    • Unigene: 29527 Drosophila melanogaster
    • Unigene: 30219 Drosophila melanogaster
    • Unigene: 30220 Drosophila melanogaster
    • Unigene: 30221 Drosophila melanogaster
    • Unigene: 30223 Drosophila melanogaster
    • Unigene: 30868 Drosophila melanogaster
    • Unigene: 30869 Drosophila melanogaster
    • Unigene: 30871 Drosophila melanogaster
    • Unigene: 30872 Drosophila melanogaster
    • Unigene: 30873 Drosophila melanogaster
    • Unigene: 30876 Drosophila melanogaster
    • Unigene: 33873 Drosophila melanogaster
    • Unigene: 5747 Drosophila melanogaster
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    see all
  • Alternative names

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

Images

  • ChIP - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    ChIP - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with 0.75% formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab177840 (blue), and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach). Primers are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    All lanes : Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840) at 1/1000 dilution

    Lanes 1 & 6 : Histone H1 Recombinant Protein
    Lanes 2 & 7 : Histone H2A Recombinant Protein
    Lanes 3 & 8 : Histone H2B Recombinant Protein
    Lanes 4 & 9 : Histone H3.1 Recombinant Protein
    Lanes 5 & 10 : Histone H4 Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    Lanes 1-5: 1% BSA blocking buffer

    Lanes 6-10: 3% Milk blocking buffer

     

    We recommend using 3% milk as the blocking agent for Western blot.

  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840) at 1/1000 dilution + Drosophila whole lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Western blot - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    All lanes : Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysates
    Lane 2 : NIH/3T3 whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Peptide Array - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Peptide Array - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    ab177840 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H4 with ab177840 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab177840 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 with ab177840 at 1/2000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of Human colon tissue is observed. Counter stained with Hematoxylin. 

    Negative control: PBS instead of primary antibody; secondary antibody is prediluted Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Histone H4 with ab177840 at 1/2000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of mouse pancreas tissue is observed. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody; secondary antibody is prediluted Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Histone H4 with ab177840 at 1/2000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on neuron cells of cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody; secondary antibody is prediluted Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
    Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)

Protocols

  • ChIP protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (11)

Publishing research using ab177840? Please let us know so that we can cite the reference in this datasheet.

ab177840 has been referenced in 11 publications.

  • Fei D  et al. KAT6A regulates stemness of aging bone marrow-derived mesenchymal stem cells through Nrf2/ARE signaling pathway. Stem Cell Res Ther 12:104 (2021). PubMed: 33541408
  • de Bont CM  et al. Neutrophil proteases degrade autoepitopes of NET-associated proteins. Clin Exp Immunol 199:1-8 (2020). PubMed: 31661552
  • Mani C  et al. The multifunctional protein PACS-1 is required for HDAC2- and HDAC3-dependent chromatin maturation and genomic stability. Oncogene 39:2583-2596 (2020). PubMed: 31988453
  • Sural S  et al. HSB-1/HSF-1 pathway modulates histone H4 in mitochondria to control mtDNA transcription and longevity. Sci Adv 6:N/A (2020). PubMed: 33087356
  • Fan H  et al. Epigenomic Reprogramming toward Mesenchymal-Epithelial Transition in Ovarian-Cancer-Associated Mesenchymal Stem Cells Drives Metastasis. Cell Rep 33:108473 (2020). PubMed: 33296650
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Schmidtea mediterranea Tissue lysate - whole (whole worm)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
10 µg
Specification
whole worm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 12 2022

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse embryonic stem cells (J1)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2018

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (MCF7 cells)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Treatment
20ng/ml TNF alpha
Specification
MCF7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ifeoluwa Adewumi

Verified customer

Submitted Feb 14 2017

Western blot abreview for Anti-Histone H4 antibody [EPR16599] - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (Recombinant octamers & purified K-562 cell histone)
Gel Running Conditions
Reduced Denaturing (18% gel)
Loading amount
0.5 µg
Specification
Recombinant octamers & purified K-562 cell histone
Blocking step
Odyssey blocking solution 1/1 (vol/vol) PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Ragnhild Eskeland

Verified customer

Submitted May 11 2015

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