Anti-HIV1 integrase antibody [IN-2] (ab66645)
![Immunocytochemistry/ Immunofluorescence - Anti-HIV1 integrase antibody [IN-2] (ab66645)](https://www.abcam.com/ps/products/66/ab66645/Images/ab66645-300492-ab6664561938.png "Immunocytochemistry/ Immunofluorescence - Anti-HIV1 integrase antibody [IN-2] (ab66645)")
Key features and details
- Mouse monoclonal [IN-2] to HIV1 integrase
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 integrase antibody [IN-2] -
Description
Mouse monoclonal [IN-2] to HIV1 integrase -
Host species
Mouse -
Specificity
ab66645 is specific for bacterial HIV1 integrase (IN) protein. -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant full length protein corresponding to HIV1 integrase. Bacterially expressed, hexahistidine amino-terminal tagged HIV1 integrase (IN) protein (clade B, HXB-3 isolate)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
IN-2 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/2000. Predicted molecular weight: 32 kDa.
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| ICC/IF | (1) |
Use at an assay dependent dilution.
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| IP | (1) |
Use at an assay dependent dilution.
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| Notes |
|---|
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WB
1/2000. Predicted molecular weight: 32 kDa. |
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ICC/IF
Use at an assay dependent dilution. |
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IP
Use at an assay dependent dilution. |
Target
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Relevance
Integrase is an enzyme, a product of the HIV1 Gag/Pol gene; the other two enzymes being reverse transcriptase and protease. The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. The HIV-1 integrase is a protein of 32 kDa and is composed of three domains. No cellular homologue of HIV integrase has been described, so potential inhibitors to this enzyme could be relatively nontoxic. -
Database links
- Entrez Gene: 155348 Human immunodeficiency virus
- SwissProt: P04585 Human immunodeficiency virus
- SwissProt: Q76353 Human immunodeficiency virus
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Alternative names
- HIV antibody
- Human Immunodeficiency virus 1 antibody
- IN antibody
- Integrase antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HIV1 integrase antibody [IN-2] (ab66645)This image is courtesy of an anonymous Abreview
ab66645 staining HIV1 integrase in transfected 293T by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody (1/2000).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab66645 has been referenced in 12 publications.
- Chia T et al. A Small Molecule, ACAi-028, with Anti-HIV-1 Activity Targets a Novel Hydrophobic Pocket on HIV-1 Capsid. Antimicrob Agents Chemother 65:e0103921 (2021). PubMed: 34228546
- Imamichi T et al. Natural Occurring Polymorphisms in HIV-1 Integrase and RNase H Regulate Viral Release and Autoprocessing. J Virol 95:e0132321 (2021). PubMed: 34523971
- Zurnic Bönisch I et al. Capsid-Labelled HIV To Investigate the Role of Capsid during Nuclear Import and Integration. J Virol 94:N/A (2020). PubMed: 31941774
- Nakamura T et al. A Conformational Escape Reaction of HIV-1 against an Allosteric Integrase Inhibitor. J Virol 94:N/A (2020). PubMed: 32611758
- Winans S & Goff SP Mutations altering acetylated residues in the CTD of HIV-1 integrase cause defects in proviral transcription at early times after integration of viral DNA. PLoS Pathog 16:e1009147 (2020). PubMed: 33351861