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Anti-HIV1 p24 antibody [5] (ab63958)

Reviews (1)Q&A (1)References (1)
Western blot - Anti-HIV1 p24 antibody [5] (ab63958)

    Key features and details

    • Mouse monoclonal [5] to HIV1 p24
    • Suitable for: ELISA, WB, ICC/IF, Radioimmunoprecipitation
    • Reacts with: Human immunodeficiency virus
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-HIV1 p24 antibody [5]
      See all HIV1 p24 primary antibodies

    • Description

      Mouse monoclonal [5] to HIV1 p24

    • Host species

      Mouse

    • Specificity

      Reacts with HIV1 native p24 as well rp24 expressed in bacteria and Cos-7 cells. Reacts with rNef of HIV1 subtype B, C and A.

    • Tested applications

      Suitable for: ELISA, WB, ICC/IF, Radioimmunoprecipitationmore details

    • Species reactivity

      Reacts with: Human immunodeficiency virus

    • Immunogen

      Synthetic peptide:

      TPQDLNTMLNTVGGH

      , corresponding to amino acids 48-62 of HIV1 HXB2 p24.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab63958 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    1/25000.
    WB (1)
    1/10000. Predicted molecular weight: 24 kDa.
    ICC/IF
    Use at an assay dependent dilution.
    Radioimmunoprecipitation
    Use at an assay dependent dilution.
    Notes
    ELISA
    1/25000.
    WB
    1/10000. Predicted molecular weight: 24 kDa.
    ICC/IF
    Use at an assay dependent dilution.
    Radioimmunoprecipitation
    Use at an assay dependent dilution.

    Target

    • Relevance

      HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

    • Cellular localization

      Membrane

    • Database links

      • Alternative names

        • CA antibody
        • Capsid protein p24 antibody
        • HIV-1 Gag p24 antibody
        • HIV1gp1 antibody
        • Human immunodeficiency virus 1 antibody
        • Human immunodeficiency virus type 1 p24 antibody
        see all

      Images

      • Western blot - Anti-HIV1 p24 antibody [5] (ab63958)
        Western blot - Anti-HIV1 p24 antibody [5] (ab63958)This image is courtesy of an anonymous Abreview
        All lanes : Anti-HIV1 p24 antibody [5] (ab63958) at 1/1000 dilution

        All lanes : Hela cells infected with HIV1 (4 hours)

        Secondary
        All lanes : Goat anti-mouse conjugated to HRP at 1/2500 dilution

        Predicted band size: 24 kDa

        See Abreview

      References (1)

      Publishing research using ab63958? Please let us know so that we can cite the reference in this datasheet.

      ab63958 has been referenced in 1 publication.

      • Yang X  et al. The neutralization of B.1.617.1 and B.1.1.529 sera from convalescent patients and BBIBP-CorV vaccines. iScience 25:105016 (2022). PubMed: 36062074

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-HIV1 p24 antibody [5]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Hela cell line)
      Loading amount
      50 µg
      Specification
      Hela cell line
      Gel Running Conditions
      Reduced Denaturing (15% TrisHCl Criterion gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 26 2008

      Question

      Catalog number(s): 9071
      Product(s): Mouse monoclonal [39/5.4A] to HIV1 p24
      Survey results
      ----------------------------------------------------------------------------------
      Telephone section
      Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
      A. No
      Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
      A.

      Protocol advice section
      Q. Did Abcam's Scientific Support give you protocol advice?
      A.Yes
      Q. How did you feel about being given protocol advice?
      A.I was not expecting to be given protocol advice, but was happy to receive this
      Q. Did the product work successfully after following the advice?
      A.No
      Q. Why have you not tried the protocol advice? (Select all that apply.)
      A.

      Free of charge replacement section
      Q. Did Abcam's Scientific Support give you a free of charge replacement?
      A. Yes

      Q. Did the replacement product work successfully?
      A.No
      Credit note / refund section
      Q. Did Abcam's Scientific Support give you a credit note or a refund?
      A.No

      Q. Would you have preferred a different outcome from receiving a credit note or refund?
      A.
      Overall satisfaction section
      Q. How satisfied are you with how the complaint was handled?
      A.Very satisfied
      Q. Could we have done anything to better resolve the problem?
      A.
      Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
      A.No, I would not mind
      Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
      A.

      Read More
      Answer

      Thank you for your participation in Abcam's post complaint survey.

      I am sorry that the replacement antibody ab63958 did not work either.

      Please let me know what problems you encountered (no bands as before?), and what antibody dilution and incubation times you used. I'd be happy to discuss this further so that we can find an acceptable solution to this problem.

      I look forward to hear back from you and assist you further.

      Read More

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