Recombinant Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)
![Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)](https://www.abcam.com/ps/products/308/ab308070/Images/ab308070-502727-anti-hiv1-p55--p17-antibody-epr27327-41-western-blot-293t-cells-transfected-with-an-empty-vector-containing-a-his-tag-whole-cell-lysate-20-ug.jpg "Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27327-41] to HIV1 p55 + p17
- Suitable for: Flow Cyt (Intra), Indirect ELISA, WB, ICC/IF
- Reacts with: Human immunodeficiency virus
Related conjugates and formulations
Overview
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Product name
Anti-HIV1 p55 + p17 antibody [EPR27327-41]
See all HIV1 p55 + p17 primary antibodies -
Description
Rabbit monoclonal [EPR27327-41] to HIV1 p55 + p17 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), Indirect ELISA, WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T cells transfected with a HIV1-GAG p55 expression vector containing a His-tag whole cell lysate. His-tagged HIV1-GAG p17 fragment. ICC/IF: HIV1-GAG expression vector containing a myc tag. Flow Cyt (Intra): HEK-293T cells transfected with Myc-tagged HIV1-GAG overexpression construct.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR27327-41 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab308070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| Indirect ELISA |
Use at an assay dependent concentration.
Use at 250 ng/ml |
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| WB |
1/1000.
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| ICC/IF |
1/2000.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
Indirect ELISA
Use at an assay dependent concentration. Use at 250 ng/ml |
|
WB
1/1000. |
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ICC/IF
1/2000. |
Target
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Relevance
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. HIV1 Gag p17, the HIV1 matrix protein is produced by the digestion of its precursor Gag p55 by HIV1 protease. -
Database links
- Entrez Gene: 155030 Human immunodeficiency virus
- SwissProt: P18800 Human immunodeficiency virus
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Alternative names
- Gag antibody
- Gag polyprotein antibody
- HIV1 matrix protein p17 antibody
see all
Images
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Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)All lanes : Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070) at 1/1000 dilution
Lane 1 : 293T cells transfected with an empty vector containing a His tag whole cell lysate
Lane 2 : 293T cells transfected with a HIV1-GAG p55 expression vector containi a His-tag whole cell lysat
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.
Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 3 seconds
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![Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)](https://www.abcam.com/ps/products/308/ab308070/Images/ab308070-502728-anti-hiv1-p55--p17-antibody-epr27327-41-western-blot-his-tagged-hiv2-gag-recombinant-protein-10-ng.jpg)
Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)All lanes : Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070) at 1/1000 dilution
Lane 1 : His-tagged HIV2-GAG recombinant protein 10 ng
Lane 2 : His-tagged HIV1-GAG p17 fragment 10 ng
Lane 3 : His-tagged HIV1-GAG p24 fragment 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.
This antibody does not cross-react with HIV2-GAG.
Exposure time: 3 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)](https://www.abcam.com/ps/products/308/ab308070/Images/ab308070-1-anti-hiv1-p55--p17-antibody-epr27327-41-immunocytochemistry-hek-293t.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling HIV1 p55 + p17 with ab308070 at 1/2000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing positive staining in HEK-293T cells transfected with a HIV1-GAG expression vector containing a myc tag and no staining in HEK-293T cells transfected with a HIV2-GAG expression vector containing a myc tag.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)](https://www.abcam.com/ps/products/308/ab308070/Images/ab308070-2-anti-hiv1-p55--p17-antibody-epr27327-41-flow-cytometry-hek-293t-cells.jpg)
Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T cells (human embryonic kidney epithelial cells) transfected with Myc-tagged HIV2-GAG overexpression construct (Left) / HEK-293T cells transfected with Myc-tagged HIV1-GAG overexpression construct (Right) labelling HIV1 p55 + p17 with ab308070 at 1/500 dilution (Right).
A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-myc conjugated to 647. -
![Indirect ELISA - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)](https://www.abcam.com/ps/products/308/ab308070/Images/ab308070-503669-ANTIHI2.JPG)
Indirect ELISA - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)Indirect ELISA analysis of ab308070 at 0-1000 ng/ml.
The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.
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![Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)](https://www.abcam.com/ps/products/308/ab308070/Images/ab308070-503668-Benefits-of-Recombinant-antibodies.jpg)
Anti-HIV1 p55 + p17 antibody [EPR27327-41] (ab308070)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab308070 has not yet been referenced specifically in any publications.