Anti-HIV1 Rev antibody [10] (ab25871)
Key features and details
- Mouse monoclonal [10] to HIV1 Rev
- Suitable for: ELISA, WB, Radioimmunoprecipitation
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 Rev antibody [10]
See all HIV1 Rev primary antibodies -
Description
Mouse monoclonal [10] to HIV1 Rev -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WB, Radioimmunoprecipitationmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
To produce this antibody the recombinant Rev (strain BRU) has been used as immunogen. The peptide sequences were not derived from particular strains of HIV1, but were designed as (artificial) B-clade (strain) consensus sequences. Sequences in Swiss prot belong to particular HIV1 strains. For epitope mapping synthetic peptides were used.
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Epitope
Epitope mapped to aa34-40 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/10000.
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WB |
1/500. Predicted molecular weight: 13 kDa.
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Radioimmunoprecipitation |
Use at an assay dependent dilution.
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Notes |
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ELISA
1/10000. |
WB
1/500. Predicted molecular weight: 13 kDa. |
Radioimmunoprecipitation
Use at an assay dependent dilution. |
Target
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Relevance
HIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection. -
Database links
- Entrez Gene: 155908 Human immunodeficiency virus
- SwissProt: P04618 Human immunodeficiency virus
- SwissProt: P69718 Human immunodeficiency virus
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Alternative names
- Human immunodeficiency virus 1 Rev antibody
- Protein Rev antibody
- Regulator of expression of viral proteins antibody
- Rev antibody
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab25871 has been referenced in 2 publications.
- Nomaguchi M et al. Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels. J Virol 90:4563-4578 (2016). PubMed: 26912631
- Nomaguchi M et al. Natural single-nucleotide polymorphisms in the 3' region of the hiv-1 pol gene modulate viral replication ability. J Virol 88:4145-60 (2014). WB . PubMed: 24478432