Recombinant Anti-HLA-C antibody [EPR26121-73] (ab307361)
![Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-499734-anti-hla-c-antibody-epr26121-73-western-blot-jurkat-human-t-cell-leukemia-t-lymphocyte-transfected-with-scrambled-sirna-control-whole-cell-lysate-20-ug.jpg "Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26121-73] to HLA-C
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HLA-C antibody [EPR26121-73]
See all HLA-C primary antibodies -
Description
Rabbit monoclonal [EPR26121-73] to HLA-C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat transfected with scrambled siRNA control, Jurkat transfected with siRNA specifically targeting HLA-C whole cell lysate, A375, HeLa, Human lymphoma, HL-60, Human colon, and HEK-293T IHC-P: Human tonsil, Human NKT lymphoma and Human colon tissues. IP: Jurkat cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26121-73 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 41 kDa.
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| IP |
1/30.
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| IHC-P |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 41 kDa. |
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IP
1/30. |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 3107 Human
- Omim: 142840 Human
- SwissProt: P04222 Human
- SwissProt: P10321 Human
- SwissProt: Q07000 Human
- Unigene: 656020 Human
- Unigene: 743218 Human
- Unigene: 77961 Human
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Alternative names
- 1C07_HUMAN antibody
- Cw-7 alpha chain antibody
- D6S204 antibody
see all
Images
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Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) transfected with scrambled siRNA control whole cell lysate 20 µg
Lane 2 : Jurkat transfected with siRNA specifically targeting HLA-C whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 26 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 26 seconds
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![Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-499735-anti-hla-c-antibody-epr26121-73-western-blot-jurkat-human-t-cell-leukemia-t-lymphocyte-whole-cell-lysate-80-ug.jpg)
Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 80 µg
Lane 2 : A375 (human malignant melanoma epithelial cell) whole cell lysate 80 µg
Lane 3 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 80 µg
Lane 4 : Human lymphoma tissue lysate 80 µg
Lane 5 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate 80 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
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![Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-499736-anti-hla-c-antibody-epr26121-73-western-blot-human-colon-tissue-lysate-40-ug.jpg)
Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)Anti-CD5 antibody [EPR26532-12] - BSA and Azide free (Detector) (ab307261) at 1/1000 dilution + Human colon tissue lysate 40 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 7.25 seconds
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![Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-499737-anti-hla-c-antibody-epr26121-73-western-blot-hek-293t-cells-transfected-with-an-empty-vector-containing-a-his-tag-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-HLA-C antibody [EPR26121-73] (ab307361)All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution
Lane 1 : HEK-293T cells transfected with an empty vector containing a his tag whole cell lysate 20 µg
Lane 2 : HEK-293T cells transfected with a human HLA-C expression vector containing a his tag whole cell lysate 20 µg
Lane 3 : HEK-293T cells transfected with a human HLA-A expression vector containing a his tag whole cell lysate 20 µg
Lane 4 : HEK-293T cells transfected with a human HLA-B expression vector containing a his tag whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human HLA-A and HLA-B.
Exposure time: 3 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-2-anti-hla-c-antibody-epr26121-73-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] (ab307361)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil (PMID: 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-3-anti-hla-c-antibody-epr26121-73-immunohistochemistry-human-nkt-lymphoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] (ab307361)Immunohistochemical analysis of paraffin-embedded Human NKT lymphoma tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on NKT lymphoma. The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-4-anti-hla-c-antibody-epr26121-73-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] (ab307361)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID: 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
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![Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-5-anti-hla-c-antibody-epr26121-73-immunoprecipitation-jurkat.jpg)
Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] (ab307361)HLA-C was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 5 ug with ab307361 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307361 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 5 ug
Lane 2: abab307361 IP in Jurkat whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab307361 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
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![Anti-HLA-C antibody [EPR26121-73] (ab307361)](https://www.abcam.com/ps/products/307/ab307361/Images/ab307361-1-benefitsofrecombinantantibodies.jpg)
Anti-HLA-C antibody [EPR26121-73] (ab307361)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab307361 has not yet been referenced specifically in any publications.
