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RecombinantRabMAb

Recombinant Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

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Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
  • Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26121-73] to HLA-C - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free
    See all HLA-C primary antibodies

  • Description

    Rabbit monoclonal [EPR26121-73] to HLA-C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat transfected with scrambled siRNA control, Jurkat transfected with siRNA specifically targeting HLA-C whole cell lysate, A375, HeLa, Human lymphoma, HL-60, Human colon, and HEK-293T IHC-P: Human tonsil, Human NKT lymphoma and Human colon tissues. IP: Jurkat cell.

  • General notes

    ab307362 is the carrier-free version of ab307361

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab307362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Information by UniProt

  • Database links

    • Alternative names

      • 1C07_HUMAN antibody
      • Cw-7 alpha chain antibody
      • D6S204 antibody
      • HLA class I histocompatibility antigen antibody
      • HLA class I histocompatibility antigen, C alpha chain antibody
      • HLA class I histocompatibility antigen, Cw-1 alpha chain antibody
      • HLA JY3 antibody
      • HLA-C antibody
      • HLA-C histocompatibility type antibody
      • HLA-Cw antibody
      • HLA-JY3 antibody
      • HLAC antibody
      • HLC C antibody
      • HLC-C antibody
      • human leukocyte antigen-C alpha chain antibody
      • major histocompatibility antigen HLA-C antibody
      • major histocompatibility complex, class I, C antibody
      • MHC class I antigen antibody
      • MHC class I antigen Cw 1 antibody
      • MHC class I antigen Cw*7 antibody
      • MHC class I antigen heavy chain HLA-C antibody
      • PSORS1 antibody
      see all

    Images

    • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution

      Lane 1 : Jurkat (human T cell leukemia T lymphocyte) transfected with scrambled siRNA control whole cell lysate 20 µg
      Lane 2 : Jurkat transfected with siRNA specifically targeting HLA-C whole cell lysate 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 26 seconds


      This data was developed using ab307361, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 26 seconds

    • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution

      Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 80 µg
      Lane 2 : A375 (human malignant melanoma epithelial cell) whole cell lysate 80 µg
      Lane 3 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 80 µg
      Lane 4 : Human lymphoma tissue lysate 80 µg
      Lane 5 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate 80 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 15 seconds


      This data was developed using ab307361, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 15 seconds

    • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution + Human colon tissue lysate 40 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 7 seconds


      This data was developed using ab307361, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 7.25 seconds

    • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution

      Lane 1 : HEK-293T cells transfected with an empty vector containing a his tag whole cell lysate 20 µg
      Lane 2 : HEK-293T cells transfected with a human HLA-C expression vector containing a his tag whole cell lysate 20 µg
      Lane 3 : HEK-293T cells transfected with a human HLA-A expression vector containing a his tag whole cell lysate 20 µg
      Lane 4 : HEK-293T cells transfected with a human HLA-B expression vector containing a his tag whole cell lysate 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 3 seconds


      This data was developed using ab307361, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      This antibody does not cross-react with human HLA-A and HLA-B.

      Exposure time: 3 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

      This data was developed using ab307361, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil (PMID: 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

      This data was developed using ab307361, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human NKT lymphoma tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on NKT lymphoma. The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

      This data was developed using ab307361, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID: 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

    • Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

      This data was developed using ab307361, the same antibody clone in a different buffer formulation.

      HLA-C was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 5 ug with ab307361 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307361 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 5 ug

      Lane 2: abab307361 IP in Jurkat whole cell lysate

      Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab307361 in Jurkat whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds

    • Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)
      Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab307362? Please let us know so that we can cite the reference in this datasheet.

    ab307362 has not yet been referenced specifically in any publications.

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