Name: Anti-HMGA2 antibody [EPR18114]
Brand: RabMAb
SKU: ab207301
Rating: 5 (1 reviews)

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Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18114] to HMGA2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Carrier Free

Product name

Anti-HMGA2 antibody [EPR18114]
See all HMGA2 primary antibodies
Description

Rabbit monoclonal [EPR18114] to HMGA2
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human gastric cancer and colon cancer tissues. ICC/IF: HeLa and HepG2 cells. WB: HEK-293, HepG2 and NCCIT cells lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18114
Isotype

IgG
Research areas

Alternative Versions
- Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
Recombinant Protein
- Recombinant Human HMGA2 protein (ab156333)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 12 kDa.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(1)	1/100.

Notes
WB 1/1000. Predicted molecular weight: 12 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Function

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
Involvement in disease

Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
Sequence similarities

Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains.
Developmental stage

Expressed predominantly during embryogenesis.
Post-translational
modifications

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
Cellular localization

Nucleus.
Target information above from: UniProt accession P52926 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 8091 Human
- Omim: 600698 Human
- SwissProt: P52926 Human
- Unigene: 505924 Human
Alternative names
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
- High mobility group (nonhistone chromosomal) protein isoform IC antibody
- High Mobility Group AT hook 2 antibody
- High Mobility Group AT hook protein 2 antibody
- High mobility group AT-hook protein 2 antibody
- High mobility group protein HMGI C antibody
- High mobility group protein HMGI-C antibody
- High mobility group protein HMGIC antibody
- High mobility group protein I, isoform C antibody
- HMGA2 antibody
- HMGA2_HUMAN antibody
- HMGI C antibody
- HMGIC antibody
- LIPO antibody
- Non histone chromosomal architectural protein HMGI C antibody
- Non histone chromosomal architectural protein HMGIC antibody
- Pygmy antibody
- STQTL9 antibody
see all

Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301)

All lanes : Anti-HMGA2 antibody [EPR18114] (ab207301) at 1/1000 dilution

Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 80 seconds

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301)

All lanes : Anti-HMGA2 antibody [EPR18114] (ab207301) at 1/1000 dilution

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) prepared in RIPA lysis method
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) prepared in RIPA lysis method
Lane 3 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) prepared in 1% SDS Hot lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon tissue. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)This image is courtesy of an abreview by Kirk Mcmanus

ab207301 staining HMGA2 in the HeLal cell line from Human Cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldahyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. ab150081 (1/200)was used as the secondary antibody.
See Abreview
Anti-HMGA2 antibody [EPR18114] (ab207301)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab207301? Please let us know so that we can cite the reference in this datasheet.

ab207301 has been referenced in 13 publications.

Ji Z et al. LINC00922 acts as a novel oncogene in gastric cancer. World J Surg Oncol 20:121 (2022). PubMed: 35428261
Liu Y et al. Effects of HMGA2 on the epithelial-mesenchymal transition-related genes in ACHN renal cell carcinoma cells-derived xenografts in nude mice. BMC Cancer 22:421 (2022). PubMed: 35439951
Ye ZH et al. LncRNA SNHG1 promotes renal cell carcinoma progression through regulation of HMGA2 via sponging miR-103a. J Clin Lab Anal 36:e24422 (2022). PubMed: 35466471
Yang S et al. Knockdown circTRIM28 enhances tamoxifen sensitivity via the miR-409-3p/HMGA2 axis in breast cancer. Reprod Biol Endocrinol 20:146 (2022). PubMed: 36180890
Takahashi T et al. Significance of HMGA2 expression as independent poor prognostic marker in perihilar and distal cholangiocarcinoma resected with curative intent. Eur J Surg Oncol 47:394-400 (2021). PubMed: 32878723

View all Publications for this product

Customer reviews and Q&As

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% Triton X-100

Specification

HeLa

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Aug 23 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Developmental stage

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (13)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-HMGA2 antibody [EPR18114]

Post-translational
modifications