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RecombinantRabMAb

Recombinant Anti-HMGA2 antibody [EPR23215-46] (ab246513)

Submit a review Submit a question References (1)
Western blot - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
  • Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
  • Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
  • Anti-HMGA2 antibody [EPR23215-46] (ab246513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23215-46] to HMGA2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HMGA2 antibody [EPR23215-46]
    See all HMGA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23215-46] to HMGA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 NCI-H1299 whole cell lysates. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab246513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/600.
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 12 kDa).
ICC/IF
1/500.
IP
1/30.
Notes
Flow Cyt (Intra)
1/600.
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 12 kDa).
ICC/IF
1/500.
IP
1/30.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.

  • Involvement in disease

    Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.

  • Sequence similarities

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.

  • Developmental stage

    Expressed predominantly during embryogenesis.

  • Post-translational
    modifications

    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • HMGA 2 antibody
      • BABL antibody
      • High mobility group (nonhistone chromosomal) protein isoform I C antibody
      • High mobility group (nonhistone chromosomal) protein isoform IC antibody
      • High Mobility Group AT hook 2 antibody
      • High Mobility Group AT hook protein 2 antibody
      • High mobility group AT-hook protein 2 antibody
      • High mobility group protein HMGI C antibody
      • High mobility group protein HMGI-C antibody
      • High mobility group protein HMGIC antibody
      • High mobility group protein I, isoform C antibody
      • HMGA2 antibody
      • HMGA2_HUMAN antibody
      • HMGI C antibody
      • HMGIC antibody
      • LIPO antibody
      • Non histone chromosomal architectural protein HMGI C antibody
      • Non histone chromosomal architectural protein HMGIC antibody
      • Pygmy antibody
      • STQTL9 antibody
      see all

    Images

    • Western blot - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
      Western blot - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
      All lanes : Anti-HMGA2 antibody [EPR23215-46] (ab246513) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 12 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 28765279)

      Exposure time: 3 minutes.

    • Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
      Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (ab246513)

      HMGA2 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate with ab246513 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246513 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug

      Lane 2: 246513 IP in HepG2 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246513 in HepG2 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

       

    • Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
      Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (ab246513)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling HMGA2 with ab246513 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
      Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (ab246513)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling HMGA2 with ab246513 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in HepG2 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    • Anti-HMGA2 antibody [EPR23215-46] (ab246513)
      Anti-HMGA2 antibody [EPR23215-46] (ab246513)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab246513? Please let us know so that we can cite the reference in this datasheet.

    ab246513 has been referenced in 1 publication.

    • He J  et al. Long Noncoding RNA CCDC144NL-AS1 Promotes the Oncogenicity of Osteosarcoma by Acting as a Molecular Sponge for microRNA-490-3p and Thereby Increasing HMGA2 Expression. Onco Targets Ther 14:1-13 (2021). PubMed: 33442262

    Customer reviews and Q&As

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