Anti-HMGCL antibody [9F5AB9] (ab110295)
![Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody [9F5AB9] (ab110295)](https://www.abcam.com/ps/products/110/ab110295/Images/HMGCL-Primary-antibodies-ab110295-1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody [9F5AB9] (ab110295)")
Key features and details
- Mouse monoclonal [9F5AB9] to HMGCL
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-HMGCL antibody [9F5AB9]
See all HMGCL primary antibodies -
Description
Mouse monoclonal [9F5AB9] to HMGCL -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells and human liver mitochondria.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
9F5AB9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 4 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 4 µg/ml. |
Target
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Function
Involved in the catabolism of branched amino acids such as leucine. -
Tissue specificity
Fibroblasts, liver and lymphoblasts. -
Pathway
Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1. -
Involvement in disease
Defects in HMGCL are the cause of 3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD) [MIM:246450]; also known as hydroxymethylglutaricaciduria or HL deficiency. An autosomal recessive disease affecting ketogenesis and L-leucine catabolism. The disease usually appears in the first year of life after a fasting period and its clinical acute symptoms include vomiting, seizures, metabolic acidosis, hypoketotic hypoglycemia and lethargy. These symptoms sometimes progress to coma, with fatal outcome in some cases. -
Sequence similarities
Belongs to the HMG-CoA lyase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3155 Human
- Omim: 246450 Human
- Omim: 613898 Human
- SwissProt: P35914 Human
- Unigene: 533444 Human
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Alternative names
- 3 hydroxy 3 methylglutaryl CoA lyase antibody
- 3 hydroxy 3 methylglutaryl Coenzyme A lyase antibody
- 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase (hydroxymethylglutaricaciduria) antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody [9F5AB9] (ab110295)Immunocytochemistry image of ab110295 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110295 at 5 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in mitochondria.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110295 has not yet been referenced specifically in any publications.