Name: Anti-HMGCS2 antibody [EPR8642]
Brand: RabMAb
SKU: ab137043
Rating: 5 (6 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8642] to HMGCS2
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Product name

Anti-HMGCS2 antibody [EPR8642]
See all HMGCS2 primary antibodies
Description

Rabbit monoclonal [EPR8642] to HMGCS2
Host species

Rabbit
Specificity

Does not cross react with HMGCS1.
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver, mouse kidney and rat kidney tissue lysates and Caco 2 cell lysate. IHC-P: Human, mouse and rat liver tissues and human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8642
Isotype

IgG
Research areas

Alternative Versions
- Anti-HMGCS2 antibody [EPR8642] - BSA and Azide free (ab240099)
- Alexa Fluor® 568 Anti-HMGCS2 antibody [EPR8642] (ab313100)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human HMGCS2 protein (ab114900)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(4)	1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
WB		1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).

Notes
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).

Application notes

Is unsuitable for Flow Cyt or ICC/IF.

Function

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Tissue specificity

High expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney.
Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Involvement in disease

Defects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency) [MIM:605911]; also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids.
Sequence similarities

Belongs to the HMG-CoA synthase family.
Cellular localization

Mitochondrion.
Target information above from: UniProt accession P54868 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3158 Human
- Entrez Gene: 15360 Mouse
- Entrez Gene: 24450 Rat
- Omim: 600234 Human
- SwissProt: P54868 Human
- SwissProt: P54869 Mouse
- SwissProt: P22791 Rat
- Unigene: 59889 Human
- Unigene: 289131 Mouse
- Unigene: 29594 Rat
see all
Alternative names
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
- 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
- 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
- HMCS2_HUMAN antibody
- HMG CoA synthase antibody
- HMG-CoA synthase antibody
- HMGCS 2 antibody
- HMGCS2 antibody
- Hydroxymethylglutaryl CoA synthase antibody
- Hydroxymethylglutaryl CoA synthase mitochondrial antibody
- Hydroxymethylglutaryl-CoA synthase antibody
- mitochondrial antibody
see all

Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM /TBST.
Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM /TBST.
Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)

All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/5000 dilution (purified)

Lane 1 : Caco-2 whole cell lysate
Lane 2 : Mouse kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)

All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal liver lysate
Lane 2 : Caco 2 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling HMGCS2 with unpurified ab137043 at a diltuion of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with unpurified ab137043 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-HMGCS2 antibody [EPR8642] (ab137043)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab137043? Please let us know so that we can cite the reference in this datasheet.

ab137043 has been referenced in 29 publications.

Gouirand V et al. Ketogenic HMG-CoA lyase and its product β-hydroxybutyrate promote pancreatic cancer progression. EMBO J 41:e110466 (2022). PubMed: 35307861
Cai D et al. Identification of the Tumor Immune Microenvironment and Therapeutic Biomarkers by a Novel Molecular Subtype Based on Aging-Related Genes in Hepatocellular Carcinoma. Front Surg 9:836080 (2022). PubMed: 35392063
Huang C et al. Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway. BMC Med 20:148 (2022). PubMed: 35422042
Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
Nishitani S et al. Ketone body 3-hydroxybutyrate enhances adipocyte function. Sci Rep 12:10080 (2022). PubMed: 35710581

View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Rat Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Liver

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2020

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Liver

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Pig Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Liver

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Liver

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Liver

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Liver

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2020

Question

We are using your antibody ab137043, cat No. 6729-1 to detect HMGCS2 protein within Human Hep G2 cultured cells, but we are not able to detect any band by western. Nevertheless, we detect significant signals of mRNAs encoding this protein. On the contrary, we are able to easily detect the protein in Caco2 cells. Therefore we wonder if your monoclonal antibody is vs. a particular isoform of this protein, including a portion which can also be absent. We see in your specifications that the antibody is produced by a synthetic peptide. Could you tell us which portion of the primary structure of the protein is covered by the peptide? Do you have other possible explanation?

Abcam community

Verified customer

Asked on Jul 12 2013

Answer

Unfortunately, we do not have a strong explanation for your observation however, I found the following summary.

http://www.proteinatlas.org/ENSG00000134240

According to this, HMGCS2 was not detectable in HepG2 whereas it was observed medium abundance of HMGCS2 in Caco-2. Therefore is would appear that this discrepancy is not due to the antibody itself but to the sample used.

Elisa Thomas

Abcam Scientific Support

Answered on Jul 12 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Pathway

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (29)

Customer reviews and Q&As

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HMGCS2 antibody [EPR8642]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HMGCS2 antibody [EPR8642]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HMGCS2 antibody [EPR8642]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HMGCS2 antibody [EPR8642]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HMGCS2 antibody [EPR8642]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HMGCS2 antibody [EPR8642]