Recombinant Anti-HMGCS2 antibody [EPR8642] (ab137043)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8642] to HMGCS2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HMGCS2 antibody [EPR8642]
See all HMGCS2 primary antibodies -
Description
Rabbit monoclonal [EPR8642] to HMGCS2 -
Host species
Rabbit -
Specificity
Does not cross react with HMGCS1. -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, mouse kidney and rat kidney tissue lysates and Caco 2 cell lysate. IHC-P: Human, mouse and rat liver tissues and human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8642 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (4) |
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).
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Notes |
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IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. -
Tissue specificity
High expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney. -
Pathway
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3. -
Involvement in disease
Defects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency) [MIM:605911]; also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids. -
Sequence similarities
Belongs to the HMG-CoA synthase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3158 Human
- Entrez Gene: 15360 Mouse
- Entrez Gene: 24450 Rat
- Omim: 600234 Human
- SwissProt: P54868 Human
- SwissProt: P54869 Mouse
- SwissProt: P22791 Rat
- Unigene: 59889 Human
see all -
Alternative names
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
- 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
see all
Images
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Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/5000 dilution (purified)
Lane 1 : Caco-2 whole cell lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : Caco 2 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 57 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling HMGCS2 with unpurified ab137043 at a diltuion of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with unpurified ab137043 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab137043 has been referenced in 29 publications.
- Gouirand V et al. Ketogenic HMG-CoA lyase and its product β-hydroxybutyrate promote pancreatic cancer progression. EMBO J 41:e110466 (2022). PubMed: 35307861
- Cai D et al. Identification of the Tumor Immune Microenvironment and Therapeutic Biomarkers by a Novel Molecular Subtype Based on Aging-Related Genes in Hepatocellular Carcinoma. Front Surg 9:836080 (2022). PubMed: 35392063
- Huang C et al. Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway. BMC Med 20:148 (2022). PubMed: 35422042
- Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
- Nishitani S et al. Ketone body 3-hydroxybutyrate enhances adipocyte function. Sci Rep 12:10080 (2022). PubMed: 35710581