Anti-HNF-4-alpha (phospho S313) antibody (ab78356)
Key features and details
- Rabbit polyclonal to HNF-4-alpha (phospho S313)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HNF-4-alpha (phospho S313) antibody
See all HNF-4-alpha primary antibodies -
Description
Rabbit polyclonal to HNF-4-alpha (phospho S313) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human HNF-4-alpha (phospho S313).
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Positive control
- Extracts from HUVEC cells. Human liver carcinoma tissue. LOVO cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/500 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
|
IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
Target
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Function
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. -
Involvement in disease
Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3172 Human
- Entrez Gene: 15378 Mouse
- Entrez Gene: 25735 Rat
- Omim: 600281 Human
- SwissProt: P41235 Human
- SwissProt: P49698 Mouse
- SwissProt: P22449 Rat
- Unigene: 116462 Human
see all -
Alternative names
- FLJ39654 antibody
- FRTS4 antibody
- Hepatic nuclear factor 4 alpha antibody
see all
Images
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Western blot - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)All lanes : Anti-HNF-4-alpha (phospho S313) antibody (ab78356) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab78356 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)Immunofluorescence analysis of LOVO cells using ab78356 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab78356 has been referenced in 1 publication.
- Ceci L et al. Knockout of the Tachykinin Receptor 1 in the Mdr2-/- (Abcb4-/-) Mouse Model of Primary Sclerosing Cholangitis Reduces Biliary Damage and Liver Fibrosis. Am J Pathol 190:2251-2266 (2020). PubMed: 32712019