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    products/primary-antibodies/hnf6-antibody-epr14976-ab186743.pdf

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Signal Transduction Metabolism Energy Metabolism
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RecombinantRabMAb

Recombinant Anti-HNF6 antibody [EPR14976] (ab186743)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)
  • Anti-HNF6 antibody [EPR14976] (ab186743)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14976] to HNF6
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Anti-HOXC8 antibody (ab79690)

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Overview

  • Product name

    Anti-HNF6 antibody [EPR14976]
    See all HNF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR14976] to HNF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, human and rat liver tissue lysates; HepG2 and Hepa1-6 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14976
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Liver development
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-HNF6 antibody [EPR14976] - BSA and Azide free (ab240965)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Notes
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
  • Tissue specificity

    Highly expressed in liver; lower expression in testis and skin.
  • Sequence similarities

    Belongs to the CUT homeobox family.
    Contains 1 CUT DNA-binding domain.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UBC0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3175 Human
    • Entrez Gene: 15379 Mouse
    • Entrez Gene: 25231 Rat
    • Omim: 604164 Human
    • SwissProt: Q9UBC0 Human
    • SwissProt: O08755 Mouse
    • SwissProt: P70512 Rat
    • Unigene: 658573 Human
    • Unigene: 303355 Mouse
    • Unigene: 48812 Rat
    see all
  • Alternative names

    • hepatocyte nuclear factor 6 alpha antibody
    • Hepatocyte nuclear factor 6 antibody
    • HNF 6 antibody
    • HNF-6 antibody
    • HNF6_HUMAN antibody
    • HNF6A antibody
    • One cut domain family member 1 antibody
    • One cut homeobox 1 antibody
    • Onecut1 antibody
    see all

Images

  • Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)
    Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)
    All lanes : Anti-HNF6 antibody [EPR14976] (ab186743) at 1/200 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
    Lane 2 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg
    Lane 3 : Rat liver tissue lysates at 15 µg
    Lane 4 : Human liver tissue lysates at 15 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 100 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    This antibody shows low affinity on liver samples which should be positive according to PMID 27445394.

  • Anti-HNF6 antibody [EPR14976] (ab186743)
    Anti-HNF6 antibody [EPR14976] (ab186743)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab186743? Please let us know so that we can cite the reference in this datasheet.

ab186743 has been referenced in 1 publication.

  • Zhong Y  et al. Tumor Supernatant Derived from Hepatocellular Carcinoma Cells Treated with Vincristine Sulfate have Therapeutic Activity. Eur J Pharm Sci N/A:105557 (2020). PubMed: 32946955

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