Recombinant Anti-HNF6 antibody [EPR14976] (ab186743)
![Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)](https://www.abcam.com/ps/products/186/ab186743/Images/ab186743-323766-anti-hnf6-antibody-epr14976-western-blot.jpg "Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14976] to HNF6
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HNF6 antibody [EPR14976]
See all HNF6 primary antibodies -
Description
Rabbit monoclonal [EPR14976] to HNF6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, human and rat liver tissue lysates; HepG2 and Hepa1-6 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14976 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. -
Tissue specificity
Highly expressed in liver; lower expression in testis and skin. -
Sequence similarities
Belongs to the CUT homeobox family.
Contains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3175 Human
- Entrez Gene: 15379 Mouse
- Entrez Gene: 25231 Rat
- Omim: 604164 Human
- SwissProt: Q9UBC0 Human
- SwissProt: O08755 Mouse
- SwissProt: P70512 Rat
- Unigene: 658573 Human
see all -
Alternative names
- hepatocyte nuclear factor 6 alpha antibody
- Hepatocyte nuclear factor 6 antibody
- HNF 6 antibody
see all
Images
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Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)All lanes : Anti-HNF6 antibody [EPR14976] (ab186743) at 1/200 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg
Lane 3 : Rat liver tissue lysates at 15 µg
Lane 4 : Human liver tissue lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 100 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
This antibody shows low affinity on liver samples which should be positive according to PMID 27445394.
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![Anti-HNF6 antibody [EPR14976] (ab186743)](https://www.abcam.com/ps/products/186/ab186743/Images/ab186743-3-benefits-of-recombinant-antibodies.png)
Anti-HNF6 antibody [EPR14976] (ab186743)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab186743 has been referenced in 1 publication.
- Zhong Y et al. Tumor Supernatant Derived from Hepatocellular Carcinoma Cells Treated with Vincristine Sulfate have Therapeutic Activity. Eur J Pharm Sci N/A:105557 (2020). PubMed: 32946955