Anti-Homeobox protein prospero antibody [P3D4-1F10] (ab201326)
![Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein prospero antibody [P3D4-1F10] (ab201326)](https://www.abcam.com/ps/products/201/ab201326/Images/ab201326-242846-anti-homeobox-protein-prospero-antibody-p3d4-1f10-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein prospero antibody [P3D4-1F10] (ab201326)")
Key features and details
- Rat monoclonal [P3D4-1F10] to Homeobox protein prospero
- Suitable for: ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG2a
Overview
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Product name
Anti-Homeobox protein prospero antibody [P3D4-1F10]
See all Homeobox protein prospero primary antibodies -
Description
Rat monoclonal [P3D4-1F10] to Homeobox protein prospero -
Host species
Rat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Drosophila stage 15 embryos.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
Purity: 97.2% -
Clonality
Monoclonal -
Clone number
P3D4-1F10 -
Isotype
IgG2a -
Light chain type
kappa
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Required for proper neuronal differentiation of most or all neurons and their precursors in central and peripheral nervous systems, axonal outgrowth and pathfinding. Not required for the specification of neuronal identity. May regulate transcription by binding to DNA. -
Tissue specificity
Expressed in embryonic neuronal precursors. Expressed in the developing CNS, lens-secreting cone cells of the eye, and midgut. -
Sequence similarities
Belongs to the Prospero homeobox family.
Contains 1 Prospero-type homeobox DNA-binding domain. -
Developmental stage
Expressed during embryogenesis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 41363 Drosophila melanogaster
- SwissProt: P29617 Drosophila melanogaster
- Unigene: 7116 Drosophila melanogaster
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Alternative names
- CG17228 antibody
- Homeobox protein prospero antibody
- Pro antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein prospero antibody [P3D4-1F10] (ab201326)
Immunohistochemistry analysis in neuroblast nuclei from Drosophila stage 15 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum. The Prospero antibody (ab201326) was at 3.4 µg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-rat at 1/400.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab201326 has not yet been referenced specifically in any publications.
