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  1. Link

    products/primary-antibodies/hoxc8-antibody-ab79690.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families HOX
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Anti-HOXC8 antibody (ab79690)

  • Datasheet
  • SDS
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Western blot - Anti-HOXC8 antibody (ab79690)
  • Immunocytochemistry/ Immunofluorescence - Anti-HOXC8 antibody (ab79690)

Key features and details

  • Rabbit polyclonal to HOXC8
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HOXC8 antibody
  • Description

    Rabbit polyclonal to HOXC8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Chicken, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human HOXC8 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101082)

  • Positive control

    • This antibody gave a positive signal in Mouse Spinal Cord and Mouse Brain Tissue Lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Developmental Biology
    • Embryogenesis
    • Axis formation and symmetry
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Bone

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities

    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P31273 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395711 Chicken
    • Entrez Gene: 3224 Human
    • Entrez Gene: 15426 Mouse
    • Omim: 142970 Human
    • SwissProt: Q9YH13 Chicken
    • SwissProt: P31273 Human
    • SwissProt: P09025 Mouse
    • Unigene: 664500 Human
    • Unigene: 6167 Mouse
    see all
  • Alternative names

    • Homeo box 3A antibody
    • Homeo box 8 antibody
    • Homeobox 3A antibody
    • Homeobox C8 antibody
    • Homeobox protein Hox C8 antibody
    • Homeobox protein Hox-3A antibody
    • Homeobox protein Hox-C8 antibody
    • Homeobox protein HoxC8 antibody
    • Homeobox protein R4 antibody
    • Homolog of mouse Hox 3.1 antibody
    • HOX 3 antibody
    • Hox 3.1 mouse homolog of antibody
    • HOX 3A antibody
    • HOX C8 antibody
    • Hox-3.1 antibody
    • Hox-3A antibody
    • HOX3 antibody
    • HOX3A antibody
    • HOXC 8 antibody
    • Hoxc-8 antibody
    • HOXC8 antibody
    • HXC8_HUMAN antibody
    • M31 antibody
    see all

Images

  • Western blot - Anti-HOXC8 antibody (ab79690)
    Western blot - Anti-HOXC8 antibody (ab79690)
    All lanes : Anti-HOXC8 antibody (ab79690) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa


    Exposure time: 16 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-HOXC8 antibody (ab79690)
    Immunocytochemistry/ Immunofluorescence - Anti-HOXC8 antibody (ab79690)
    ICC/IF image of ab79690 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79690, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab79690? Please let us know so that we can cite the reference in this datasheet.

ab79690 has been referenced in 1 publication.

  • Wang AH  et al. Down-regulation of long non-coding RNA HOTAIR inhibits invasion and migration of oesophageal cancer cells via up-regulation of microRNA-204. J Cell Mol Med 23:6595-6610 (2019). PubMed: 31389660

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