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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

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Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
  • Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
  • Flow Cytometry (Intracellular) - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
  • Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10466(B)] to HP1 gamma/CBX3
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HP1 gamma/CBX3 antibody [EPR10466(B)]
    See all HP1 gamma/CBX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10466(B)] to HP1 gamma/CBX3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, MOLT4 and HeLa cell lysates; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

  • Sequence similarities

    Contains 2 chromo domains.

  • Post-translational
    modifications

    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

  • Cellular localization

    Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

Images

  • Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
    Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
    Lane 3: NIH3T3 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab154195 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab154195 was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154195 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
    Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
    All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 20 kDa

  • Flow Cytometry (Intracellular) - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
    Flow Cytometry (Intracellular) - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

    Intracellular flow cytometric analysis of permeabilized HeLa cells labeling HP1 gamma/CBX3 with ab154195 at 1/10 dilution (red) compared with a rabbit IgG negative control antibody (green). 

     

     

  • Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)
    Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

References (0)

Publishing research using ab154195? Please let us know so that we can cite the reference in this datasheet.

ab154195 has not yet been referenced specifically in any publications.

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