Anti-HPV18 E7 antibody [8E2] (ab100953)
![Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)](https://www.abcam.com/ps/products/100/ab100953/Images/ab100953-114068-anti-hpv18-e7-antibody-8e2-western-blot.jpg "Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)")
Key features and details
- Mouse monoclonal [8E2] to HPV18 E7
- Suitable for: WB, IP, ELISA, ICC/IF
- Reacts with: Human papillomavirus
- Isotype: IgG1
Overview
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Product name
Anti-HPV18 E7 antibody [8E2]
See all HPV18 E7 primary antibodies -
Description
Mouse monoclonal [8E2] to HPV18 E7 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human papillomavirus -
Immunogen
Recombinant full length HPV18 E7 protein
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Epitope
Mapped to amino acids 36-70 -
Positive control
- WB: U20S, Hela and SiHa cell lysates. ICC/IF: U2OS cells transfected with plasmid expressing HPV18 E7 protein; Hela cells.
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General notes
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8E2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 12 kDa.
|
| IP |
1/500.
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|
| ELISA |
1/128000.
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| ICC/IF |
Use at an assay dependent dilution.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 12 kDa. |
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IP
1/500. |
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ELISA
1/128000. |
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ICC/IF
Use at an assay dependent dilution. |
Target
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Relevance
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle. -
Database links
- SwissProt: P06788 Human papillomavirus
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Alternative names
- E7 protein antibody
- Human Papilloma Virus Type 18 E7 protein antibody
Images
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Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)All lanes : Anti-HPV18 E7 antibody [8E2] (ab100953) at 1/1000 dilution
Lane 1 : lysate of U20S cells with plasmid expressing E7 protein
Lane 2 : plain U20S lysate
Lane 3 : Hela lysate. HeLa cells containing HPV-18 genome sequences expressing HPV E6 and E7 proteins.
Lane 4 : SiHa lysate
Lane 5 : Marker
Predicted band size: 12 kDa -
![Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)](https://www.abcam.com/ps/products/100/ab100953/Images/ab100953-114067-anti-hpv18-e7-antibody-8e2-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)IP analysis of HPV18 E7 protein using ab100953 at 1/500:
Lane 1: detection of E7 protein in lysate of U20S cells
Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of antibody
Lane 3: precipitation without ab100953
Lane 4: IP exp. with plain U2OS cells
Lane 5: IP exp. with non-transfected HeLa cells. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells.
Lane 6: marker. -
![Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)](https://www.abcam.com/ps/products/100/ab100953/Images/ab100953-114066-anti-hpv18-e7-antibody-8e2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab100953 has been referenced in 16 publications.
- Sun X et al. Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes. Int J Mol Med 47:335-345 (2021). PubMed: 33236130
- Morgan EL et al. E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer. Cell Death Differ 28:1669-1687 (2021). PubMed: 33303976
- Chen Y et al. In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system. Antiviral Res 178:104794 (2020). PubMed: 32298665
- Morgan EL et al. MicroRNA-18a targeting of the STK4/MST1 tumour suppressor is necessary for transformation in HPV positive cervical cancer. PLoS Pathog 16:e1008624 (2020). PubMed: 32555725
- Ding X et al. A DHX9-lncRNA-MDM2 interaction regulates cell invasion and angiogenesis of cervical cancer. Cell Death Differ 26:1750-1765 (2019). PubMed: 30518908