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  1. Link

    products/primary-antibodies/hpv18-e7-antibody-8e2-ab100953.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Human Papillomavirus
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Anti-HPV18 E7 antibody [8E2] (ab100953)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (16)

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Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)
  • Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)
  • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)

Key features and details

  • Mouse monoclonal [8E2] to HPV18 E7
  • Suitable for: WB, IP, ELISA, ICC/IF
  • Reacts with: Human papillomavirus
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
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Recombinant Human papillomavirus HPV18 E7 protein (His tag) (ab236931)

View more associated products

Overview

  • Product name

    Anti-HPV18 E7 antibody [8E2]
    See all HPV18 E7 primary antibodies
  • Description

    Mouse monoclonal [8E2] to HPV18 E7
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human papillomavirus
  • Immunogen

    Recombinant full length HPV18 E7 protein

  • Epitope

    Mapped to amino acids 36-70
  • Positive control

    • WB: U20S, Hela and SiHa cell lysates. ICC/IF: U2OS cells transfected with plasmid expressing HPV18 E7 protein; Hela cells.
  • General notes

    Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    8E2
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Human Papillomavirus
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • HPV

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human papillomavirus HPV18 E7 protein (His tag) (ab236931)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab100953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Predicted molecular weight: 12 kDa.
IP
1/500.
ELISA
1/128000.
ICC/IF
Use at an assay dependent dilution.
Notes
WB
1/1000. Predicted molecular weight: 12 kDa.
IP
1/500.
ELISA
1/128000.
ICC/IF
Use at an assay dependent dilution.

Target

  • Relevance

    Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
  • Database links

    • SwissProt: P06788 Human papillomavirus
    • Alternative names

      • E7 protein antibody
      • Human Papilloma Virus Type 18 E7 protein antibody

    Images

    • Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)
      Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)
      All lanes : Anti-HPV18 E7 antibody [8E2] (ab100953) at 1/1000 dilution

      Lane 1 : lysate of U20S cells with plasmid expressing E7 protein
      Lane 2 : plain U20S lysate
      Lane 3 : Hela lysate. HeLa cells containing HPV-18 genome sequences expressing HPV E6 and E7 proteins.
      Lane 4 : SiHa lysate
      Lane 5 : Marker

      Predicted band size: 12 kDa

    • Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)
      Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)

      IP analysis of HPV18 E7 protein using ab100953 at 1/500:
      Lane 1: detection of E7 protein in lysate of U20S cells
      Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of antibody
      Lane 3: precipitation without ab100953
      Lane 4: IP exp. with plain U2OS cells
      Lane 5: IP exp. with non-transfected HeLa cells. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells.
      Lane 6: marker.

    • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)
      Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)
      Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.

    Protocols

    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab100953? Please let us know so that we can cite the reference in this datasheet.

    ab100953 has been referenced in 16 publications.

    • Sun X  et al. Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes. Int J Mol Med 47:335-345 (2021). PubMed: 33236130
    • Morgan EL  et al. E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer. Cell Death Differ 28:1669-1687 (2021). PubMed: 33303976
    • Chen Y  et al. In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system. Antiviral Res 178:104794 (2020). PubMed: 32298665
    • Morgan EL  et al. MicroRNA-18a targeting of the STK4/MST1 tumour suppressor is necessary for transformation in HPV positive cervical cancer. PLoS Pathog 16:e1008624 (2020). PubMed: 32555725
    • Ding X  et al. A DHX9-lncRNA-MDM2 interaction regulates cell invasion and angiogenesis of cervical cancer. Cell Death Differ 26:1750-1765 (2019). PubMed: 30518908
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-HPV18 E7 antibody [8E2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (human foreskin keratinocytes)
    Loading amount
    10 µg
    Specification
    human foreskin keratinocytes
    Gel Running Conditions
    Reduced Denaturing (15% Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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