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RecombinantRabMAb

Recombinant HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)

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Western blot - HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)
  • HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR1172Y] to 4-1BBL
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-4-1BBL antibody [EPR1172Y]
    See all 4-1BBL primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR1172Y] to 4-1BBL

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).
Notes
WB
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

  • Tissue specificity

    Expressed in brain, placenta, lung, skeletal muscle and kidney.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 4 1BB L antibody
      • 4 1BB ligand antibody
      • 4 1BBL antibody
      • 4-1BB ligand antibody
      • 4-1BBL antibody
      • Cd137l antibody
      • Cd157l antibody
      • Homolog of mouse 4 1BB L antibody
      • Homolog of mouse 4 1BBL antibody
      • ILA ligand (TNF related) antibody
      • Ly63l antibody
      • Receptor 4 1BB ligand antibody
      • TNF superfamily member 9 antibody
      • TNFL9_HUMAN antibody
      • Tnfsf9 antibody
      • TNLG5A antibody
      • Tumor necrosis factor (ligand) superfamily member 9 antibody
      • Tumor necrosis factor ligand 5A antibody
      • Tumor necrosis factor ligand superfamily member 9 antibody
      • Tumor necrosis factor superfamily member 9 antibody
      see all

    Images

    • Western blot - HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)
      Western blot - HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)
      HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523) at 1/5000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 27 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 12 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199523 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)
      HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)

    References (0)

    Publishing research using ab199523? Please let us know so that we can cite the reference in this datasheet.

    ab199523 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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