Recombinant HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
![Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)](https://www.abcam.com/ps/products/237/ab237339/Images/ab237339-454137-HRP-Anti6X-His-tag-antibody.jpg "Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR20547] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-6X His tag® antibody [EPR20547]
See all 6X His tag® primary antibodies -
Description
HRP Rabbit monoclonal [EPR20547] to 6X His tag® -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20547 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)
- Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (ab237336)
- Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
- PE Anti-6X His tag® antibody [EPR20547] (ab237338)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/50000.
|
| Notes |
|---|
|
WB
1/5000 - 1/50000. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)All lanes : HRP Anti-6X His tag® antibody [EPR20547] (ab237339) at 1/50000 dilution
Lane 1 : HEK-293 transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag
Lysates/proteins at 15 µg per lane.
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
![Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)](https://www.abcam.com/ps/products/237/ab237339/Images/ab237339-387589-anti-6x-his-tag-antibody-epr20547-hrp-western-blot-histone-h3-recombinat-ab198757-4-minute-exposure-empty-histone-h4-recombinant-ab198115.jpg)
Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339)All lanes : HRP Anti-6X His tag® antibody [EPR20547] (ab237339) at 1/5000 dilution
Lane 1 : Histone H3 recombinant (ab198757) (4 minute exposure) cell lysate
Lane 2 : Histone H4 recombinant (ab198115) (1 minute 30 seconds exposure) cell lysate
Lane 3 : Histone H2A recombinant (ab200295)(1 minute 30 seconds exposure) cell lysate
Lysates/proteins at 2 µg per lane.
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?ab237339 was shown to react with 6X His tag® in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab237339 overnight at 4°C at a 1 in 5000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
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![HRP Anti-6X His tag® antibody [EPR20547] (ab237339)](https://www.abcam.com/ps/products/237/ab237339/Images/ab237339-2-benefits-of-recombinant-antibodies.png)
HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab237339 has been referenced in 1 publication.
- Xu Y et al. A small Rho GTPase OsRacB is required for pollen germination in rice. Dev Growth Differ 64:88-97 (2022). PubMed: 34519039