HRP Anti-beta Actin antibody [AC-15] (ab49900)
![Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)](https://www.abcam.com/ps/products/49/ab49900/Images/ab49900-210129-anti-beta-actin-antibody-ac-15-hrp-western-blot.jpg "Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)")
Key features and details
- HRP Mouse monoclonal [AC-15] to beta Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, African green monkey
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
HRP Anti-beta Actin antibody [AC-15]
See all beta Actin primary antibodies -
Description
HRP Mouse monoclonal [AC-15] to beta Actin -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, African green monkey
Predicted to work with: a wide range of other species
Does not react with:
Drosophila melanogaster, Dictyostelium discoideum -
Immunogen
Synthetic peptide corresponding to beta Actin (N terminal) conjugated to keyhole limpet haemocyanin.
Sequence:DDDIAALVIDNGSGK
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Positive control
- NIH 3T3 whole cell lysate (ab7179) can be used as a positive control in WB. Chicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts. Rat dorsal root ganglion and Rat liver lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AC-15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Positive Controls
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Recombinant Protein
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Related Products
- Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
- Human beta Actin peptide (ab13772)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (10) |
1/25000 - 1/50000. Predicted molecular weight: 42 kDa.
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| Notes |
|---|
|
WB
1/25000 - 1/50000. Predicted molecular weight: 42 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60712 Cow
- SwissProt: O18840 Dog
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)All lanes : HRP Anti-beta Actin antibody [AC-15] (ab49900) at 1/50000 dilution
Lane 1 : A549 Lysate
Lane 2 : HeLa Lysate
Lane 3 : 3T3 Lysate
Lane 4 : NRK Lysate
Lane 5 : MDCK Lysate
Lane 6 : MDBK Lysate
Lane 7 : BHK Lysate
Lane 8 : COS7 Lysate
Lane 9 : CFB Lysate
Developed using the ECL technique.
Predicted band size: 42 kDa -
![Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)](https://www.abcam.com/ps/products/49/ab49900/Images/ab49900-260057-anti-beta-actin-antibody-ac-15-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 (ab6672), anti- IL-17 at 1:3000, anti-TGF-β at 1:1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.
Normalization of results was ensured by running parallel western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.
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![Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)](https://www.abcam.com/ps/products/49/ab49900/Images/ab49900-61916-anti-beta-actin-antibody-ac-15-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)This image is courtesy of an anonymous AbreviewHRP Anti-beta Actin antibody [AC-15] (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Blocking Step: 5% Milk for 1 hour at 22°C.
Datasheets and documents
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SDS download
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Datasheet download
References (395)
ab49900 has been referenced in 395 publications.
- Bagdanoff JT et al. Clearance of plasma PCSK9 via the asialoglycoprotein receptor mediated by heterobifunctional ligands. Cell Chem Biol 30:97-109.e9 (2023). PubMed: 36626903
- Klouda T et al. Interferon-alpha or -beta facilitates SARS-CoV-2 pulmonary vascular infection by inducing ACE2. Angiogenesis 25:225-240 (2022). PubMed: 34714440
- Bechara R et al. The RNA-binding protein IMP2 drives a stromal-Th17 cell circuit in autoimmune neuroinflammation. JCI Insight 7:N/A (2022). PubMed: 34914635
- Ma L et al. FLT4/VEGFR3 activates AMPK to coordinate glycometabolic reprogramming with autophagy and inflammasome activation for bacterial elimination. Autophagy 18:1385-1400 (2022). PubMed: 34632918
- Graham GT et al. Comprehensive profiling of mRNA splicing indicates that GC content signals altered cassette exon inclusion in Ewing sarcoma. NAR Cancer 4:zcab052 (2022). PubMed: 35047826