For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/hrp-beta-actin-antibody-ac-15-ab49900.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Isotype/Loading Controls Loading Controls Beta Actin
Share by email

HRP Anti-beta Actin antibody [AC-15] (ab49900)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (322)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)

Key features and details

  • HRP Mouse monoclonal [AC-15] to beta Actin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, African green monkey
  • Conjugation: HRP
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC Unconjugated

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Primary
Product image
Anti-nNOS (neuronal) antibody - N-terminal (ab229785)
Protein
Product image
Recombinant Human beta Actin protein (His tag) (ab240844)

View more associated products

Overview

  • Product name

    HRP Anti-beta Actin antibody [AC-15]
    See all beta Actin primary antibodies
  • Description

    HRP Mouse monoclonal [AC-15] to beta Actin
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, African green monkey
    Predicted to work with: a wide range of other speciesDoes not react with: Drosophila melanogaster, Dictyostelium discoideum
  • Immunogen

    Synthetic peptide corresponding to beta Actin (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    DDDIAALVIDNGSGK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • NIH 3T3 whole cell lysate (ab7179) can be used as a positive control in WB. Chicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts. Rat dorsal root ganglion and Rat liver lysates
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    AC-15
  • Isotype

    IgG1
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Beta Actin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Alternative Versions

    • Anti-beta Actin antibody [AC-15] (ab6276)
    • FITC Anti-beta Actin antibody [AC-15] (ab6277)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Human beta Actin peptide (ab13772)
    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab49900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (10)
1/25000 - 1/50000. Predicted molecular weight: 42 kDa.
Notes
WB
1/25000 - 1/50000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280979 Cow
    • Entrez Gene: 403580 Dog
    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 81822 Rat
    • Omim: 102630 Human
    • SwissProt: P60712 Cow
    • SwissProt: O18840 Dog
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: P60711 Rat
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    see all
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    All lanes : HRP Anti-beta Actin antibody [AC-15] (ab49900) at 1/50000 dilution

    Lane 1 : A549 Lysate
    Lane 2 : HeLa Lysate
    Lane 3 : 3T3 Lysate
    Lane 4 : NRK Lysate
    Lane 5 : MDCK Lysate
    Lane 6 : MDBK Lysate
    Lane 7 : BHK Lysate
    Lane 8 : COS7 Lysate
    Lane 9 : CFB Lysate

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 (ab6672), anti- IL-17 at 1:3000, anti-TGF-β at 1:1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.

    Normalization of results was ensured by running parallel western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.

  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)This image is courtesy of an anonymous Abreview
    HRP Anti-beta Actin antibody [AC-15] (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa



    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (322)

Publishing research using ab49900? Please let us know so that we can cite the reference in this datasheet.

ab49900 has been referenced in 322 publications.

  • Jefferys SR  et al. Epigenomic characterization of latent HIV infection identifies latency regulating transcription factors. PLoS Pathog 17:e1009346 (2021). PubMed: 33635929
  • Wang B  et al. circNRIP1 facilitates keloid progression via FXR1-mediated upregulation of miR-503-3p and miR-503-5p. Int J Mol Med 47:N/A (2021). PubMed: 33649815
  • González-Calvo I  et al. Sushi domain-containing protein 4 controls synaptic plasticity and motor learning. Elife 10:N/A (2021). PubMed: 33661101
  • Baraghithy S  et al. Renal Proximal Tubule Cell Cannabinoid-1 Receptor Regulates Bone Remodeling and Mass via a Kidney-to-Bone Axis. Cells 10:N/A (2021). PubMed: 33671138
  • Lehman CW  et al. Resveratrol Inhibits Venezuelan Equine Encephalitis Virus Infection by Interfering with the AKT/GSK Pathway. Plants (Basel) 10:N/A (2021). PubMed: 33673026
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 10 Abreviews or Q&A

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Fat-adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2016

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Fat-adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2016

Western blot abreview for Anti-beta Actin [AC-15] antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 08 2014

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (ParC5 rat parotid cell line)
Loading amount
50 µg
Specification
ParC5 rat parotid cell line
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 28 2010

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
10 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 19 2010

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 20 2007

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 20 2007

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
30 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 20 2007

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
30 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 21 2007

Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
20 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.