HRP Anti-beta Actin antibody [BA3R] (ab173838)
![Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)](https://www.abcam.com/ps/products/173/ab173838/Images/ab173838-197657-anti-beta-actin-antibody-ba3r-hrp-western-blot.jpg "Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)")
Key features and details
- HRP Mouse monoclonal [BA3R] to beta Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-beta Actin antibody [BA3R]
See all beta Actin primary antibodies -
Description
HRP Mouse monoclonal [BA3R] to beta Actin -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human beta Actin (N terminal).
Database link: P60709 -
Positive control
- WB: HeLa, 293T, U2OS, COS7, NIH 3T3, NRK, A549, MDCK, C2C12, MDA-MB-231 and RSC96 cell lysates. Mouse lung tissue extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
BA3R -
Isotype
IgG2b -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Recombinant Protein
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 42 kDa.Can be blocked with Recombinant Human beta Actin protein (His tag) (ab240844).
|
| Notes |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 42 kDa.Can be blocked with Recombinant Human beta Actin protein (His tag) (ab240844). |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: O18840 Dog
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)All lanes : HRP Anti-beta Actin antibody [BA3R] (ab173838) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U2OS cell lysate
Lane 4 : COS7 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lane 6 : NRK cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa4-20% Tris-HCl polyacrylamide gel.
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![Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)](https://www.abcam.com/ps/products/173/ab173838/Images/ab173838-467657-hrp-anti-beta-actin-antibody-ba3r-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)All lanes : HRP Anti-beta Actin antibody [BA3R] (ab173838) at 1 µg
Lane 1 : A549 (Human alveolar basal epithelial cells)
Lane 2 : COS-7 (African green monkey kidney fibroblast cells)
Lane 3 : MDCK (Madin-Darby Canine Kidney cells)
Lane 4 : C2C12 (Mouse myoblast cells)
Lane 5 : MDA-MB-231 (Human breast cancer cells)
Lane 6 : RSC96
Lane 7 : Mouse lung tissue lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking buffer: 5% milk.
Datasheets and documents
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Datasheet download
References (7)
ab173838 has been referenced in 7 publications.
- Wang T et al. A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells. Stem Cell Res Ther 11:365 (2020). PubMed: 32831139
- Wang T et al. Small RNA sequencing reveals a novel tsRNA-06018 playing an important role during adipogenic differentiation of hMSCs. J Cell Mol Med 24:12736-12749 (2020). PubMed: 32939933
- Pan Y et al. Bromodomain PHD-finger transcription factor promotes glioma progression and indicates poor prognosis. Oncol Rep 41:246-256 (2019). PubMed: 30542695
- Wang T et al. Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells. Mol Med Rep 19:4719-4726 (2019). PubMed: 31059010
- Canzoneri R et al. Alternative splicing variant of RHBDD2 is associated with cell stress response and breast cancer progression. Oncol Rep 40:909-915 (2018). PubMed: 29901166
- Li X et al. miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR. Mol Cell Biochem N/A:N/A (2018). PubMed: 29959592
- Windrem MS et al. Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia. Cell Stem Cell 21:195-208.e6 (2017). PubMed: 28736215
