HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29577-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)")
Key features and details
- HRP Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control
See all beta Actin primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Drosophila melanogaster, Monkey, African green monkey, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)).
(Peptide available asab13772) -
Positive control
- HeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam 8226 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092)
- Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576)
- Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free (ab264083)
- Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
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Immunizing Peptide (Blocking)
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Recombinant Protein
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- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (17) |
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.
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| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 100033878 Horse
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : human testis lysate
Lane 2 : human brain lysate
Lane 3 : human tonsil lysate
Lane 4 : mouse brain lysate
Lane 5 : mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Exposure time: 2 minutes -
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29576-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : 3T3 whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds -
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29575-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)Lanes 1-4 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
Lanes 5-8 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% MILK)
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lanes 3 & 7 :NIH/3T3 whole cell lysate (ab7179)
Lanes 4 & 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
Datasheets and documents
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SDS download
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Datasheet download
References (235)
ab20272 has been referenced in 235 publications.
- Uysal-Onganer P et al. Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures. Int J Mol Sci 22:N/A (2021). PubMed: 33573274
- Li Y et al. Transcriptome profiling of long noncoding RNAs and mRNAs in spinal cord of a rat model of paclitaxel-induced peripheral neuropathy identifies potential mechanisms mediating neuroinflammation and pain. J Neuroinflammation 18:48 (2021). PubMed: 33602238
- Klämbt V et al. Generation of Monogenic Candidate Genes for Human Nephrotic Syndrome Using 3 Independent Approaches. Kidney Int Rep 6:460-471 (2021). PubMed: 33615071
- He MX et al. Transcriptional mediators of treatment resistance in lethal prostate cancer. Nat Med 27:426-433 (2021). PubMed: 33664492
- Du J et al. Arid1a-Plagl1-Hh signaling is indispensable for differentiation-associated cell cycle arrest of tooth root progenitors. Cell Rep 35:108964 (2021). PubMed: 33826897