HRP Anti-beta Tubulin antibody [BT7R] (ab173840)
Key features and details
- HRP Mouse monoclonal [BT7R] to beta Tubulin
- Suitable for: WB
- Reacts with: Mouse, Rat, Dog, Human, Non human primates, African green monkey
- Conjugation: HRP
- Isotype: IgG2a
Overview
-
Product name
HRP Anti-beta Tubulin antibody [BT7R]
See all beta Tubulin primary antibodies -
Description
HRP Mouse monoclonal [BT7R] to beta Tubulin -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human, Non human primates, African green monkey -
Immunogen
Synthetic peptide corresponding to Human beta Tubulin (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P07437 -
Positive control
- WB: A431, U2OS, 293T, Jurkat, HeLa, COS-7, C2C12, NRK, A549, MDCK, MDA-MB-231, PC-12 and RSC96 cell lysates. Mouse lung tissue extracts.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 0.07% Kathon, 99.93% PBS -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
BT7R -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Positive Controls
-
Recombinant Protein
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
|
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
Target
-
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Tissue specificity
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
Involvement in disease
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 203068 Human
- Entrez Gene: 22154 Mouse
- Entrez Gene: 29214 Rat
- Omim: 191130 Human
- SwissProt: P07437 Human
- SwissProt: P99024 Mouse
- SwissProt: P69897 Rat
- Unigene: 636480 Human
-
Alternative names
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
Images
-
All lanes : HRP Anti-beta Tubulin antibody [BT7R] (ab173840) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : U2OS cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : COS7 cell lysate
Lane 7 : C2C12 cell lysate
Lane 8 : NRK cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa4-20% Tris-HCl polyacrylamide gel.
-
All lanes : HRP Anti-beta Tubulin antibody [BT7R] (ab173840) at 1 µg/ml
Lane 1 : A549 (Human adenocarcinomic alveolar basal epithelial cells)
Lane 2 : COS-7 (African green monkey kidney fibroblast cells)
Lane 3 : MDCK (Madin-Darby Canine Kidney cells)
Lane 4 : C2C12 (Mouse myoblast cells)
Lane 5 : MDA-MB-231 (Human breast cancer cells)
Lane 6 : PC-12 (Embryonic Rat adrenal medulla cells)
Lane 7 : RSC96
Lane 8 : Mouse lung tissue
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking buffer: 5% milk.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab173840 has been referenced in 1 publication.
- Chen C et al. Proteomic analysis of the effects of CSF-1 and IL-1a on dental follicle cells. Mol Med Rep 14:2405-14 (2016). PubMed: 27484316