Recombinant HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)](https://www.abcam.com/ps/products/309/ab309163/Images/ab309163-1-anti-cardiac-troponin-i-antibody-epr20307-immunohistochemistry-human-cardiac-muscle1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR20307] to Cardiac Troponin I
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Cardiac Troponin I antibody [EPR20307]
See all Cardiac Troponin I primary antibodies -
Description
HRP Rabbit monoclonal [EPR20307] to Cardiac Troponin I -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human cardiac muscle, Mouse cardiac muscle, Rat cardiac muscle
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20307 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab309163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the troponin I family. - Information by UniProt
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Database links
- Entrez Gene: 7137 Human
- Entrez Gene: 21954 Mouse
- Entrez Gene: 29248 Rat
- Omim: 191044 Human
- SwissProt: P19429 Human
- SwissProt: P48787 Mouse
- SwissProt: P23693 Rat
- Unigene: 709179 Human
see all -
Alternative names
- cardiac muscle antibody
- Cardiac troponin I antibody
- cardiomyopathy, dilated 2A (autosomal recessive) antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Cardiac Troponin I with ab309163 at 1/100 (5.0 ug/ml). Positive staining on human cardiac muscle. The section was incubated with ab309163 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)](https://www.abcam.com/ps/products/309/ab309163/Images/ab309163-2-anti-cardiac-troponin-i-antibody-epr20307-immunohistochemistry-mouse-cardiac-muscle1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Cardiac Troponin I with ab309163 at 1/100 (5.0 ug/ml). Positive staining on mouse cardiac muscle. The section was incubated with ab309163 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)](https://www.abcam.com/ps/products/309/ab309163/Images/ab309163-3-anti-cardiac-troponin-i-antibody-epr20307-immunohistochemistry-rat-cardiac-muscle1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Cardiac Troponin I with ab309163 at 1/100 (5.0 ug/ml). Positive staining on rat cardiac muscle. The section was incubated with ab309163 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)](https://www.abcam.com/ps/products/309/ab309163/Images/ab309163-6-anti-cardiac-troponin-i-antibody-epr20307-immunohistochemistry-human-skeletal-muscl1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Cardiac Troponin I with ab309163 at 1/100 (5.0 μg/ml). Negative control: no staining on human skeletal muscle. The section was incubated with ab309163 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)](https://www.abcam.com/ps/products/309/ab309163/Images/ab309163-8-anti-cardiac-troponin-i-antibody-epr20307-immunohistochemistry-mouse-skeletal-muscl1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Cardiac Troponin I with ab309163 at 1/100 (5.0 μg/ml). Negative control: no staining on mouse skeletal muscle. The section was incubated with ab309163 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)](https://www.abcam.com/ps/products/309/ab309163/Images/ab309163-4-anti-cardiac-troponin-i-antibody-epr20307-immunohistochemistry-rat-skeletal-muscle1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Cardiac Troponin I with ab309163 at 1/100 (5.0 ug/ml). Negative control: no staining on rat skeletal muscle. The section was incubated with ab309163 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)](https://www.abcam.com/ps/products/309/ab309163/Images/ab309163-7-Benefits-of-Recombinant-antibodies.jpg)
HRP Anti-Cardiac Troponin I antibody [EPR20307] (ab309163)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab309163 has not yet been referenced specifically in any publications.