HRP Anti-CD68 antibody [FA-11] (ab216526)
![Western blot - HRP Anti-CD68 antibody [FA-11] (ab216526)](https://www.abcam.com/ps/products/216/ab216526/Images/ab216526-284029-anti-cd68-antibody-fa-11-hrp-western-blot.jpg "Western blot - HRP Anti-CD68 antibody [FA-11] (ab216526)")
Key features and details
- HRP Rat monoclonal [FA-11] to CD68
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
HRP Anti-CD68 antibody [FA-11]
See all CD68 primary antibodies -
Description
HRP Rat monoclonal [FA-11] to CD68 -
Host species
Rat -
Conjugation
HRP -
Specificity
Detects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raw264.7
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
FA-11 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
- Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
- Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
- PE Anti-CD68 antibody [FA-11] (ab216701)
- PerCP Anti-CD68 antibody [FA-11] (ab220509)
- APC Anti-CD68 antibody [FA-11] (ab221251)
- Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
- Anti-CD68 antibody [FA-11] (ab53444)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 12514 Mouse
- SwissProt: P31996 Mouse
- Unigene: 15819 Mouse
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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Western blot - HRP Anti-CD68 antibody [FA-11] (ab216526)All lanes : HRP Anti-CD68 antibody [FA-11] (ab216526) at 1/5000 dilution
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : RAW 264.7 LPS treated (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Additional bands at: 100 kDa (possible glycosylated form)
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab216526 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216526 has not yet been referenced specifically in any publications.