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    products/primary-antibodies/hrp-cd68-antibody-fa-11-ab216526.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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HRP Anti-CD68 antibody [FA-11] (ab216526)

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Western blot - HRP Anti-CD68 antibody [FA-11] (ab216526)

    Key features and details

    • HRP Rat monoclonal [FA-11] to CD68
    • Suitable for: WB
    • Reacts with: Mouse
    • Conjugation: HRP
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free PE PerCP Unconjugated

    You may also be interested in

    Protein
    Recombinant human CD68 protein (Fc Chimera Active) (ab214829)
    Primary
    Product image
    Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
    Primary
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    Anti-CD68 antibody [FA-11] (ab53444)

    View more associated products

    Overview

    • Product name

      HRP Anti-CD68 antibody [FA-11]
      See all CD68 primary antibodies
    • Description

      HRP Rat monoclonal [FA-11] to CD68
    • Host species

      Rat
    • Conjugation

      HRP
    • Specificity

      Detects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • Raw264.7
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Proclin 300 Solution
      Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      FA-11
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Neuroscience
      • Development

    Associated products

    • Alternative Versions

      • Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
      • Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
      • Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
      • PE Anti-CD68 antibody [FA-11] (ab216701)
      • PerCP Anti-CD68 antibody [FA-11] (ab220509)
      • APC Anti-CD68 antibody [FA-11] (ab221251)
      • Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
      • Anti-CD68 antibody [FA-11] (ab53444)
    • Recombinant Protein

      • Recombinant human CD68 protein (Fc Chimera Active) (ab214829)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab216526 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.

    Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similarities

      Belongs to the LAMP family.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Target information above from: UniProt accession P34810 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12514 Mouse
      • SwissProt: P31996 Mouse
      • Unigene: 15819 Mouse
      • Alternative names

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Western blot - HRP Anti-CD68 antibody [FA-11] (ab216526)
        Western blot - HRP Anti-CD68 antibody [FA-11] (ab216526)
        All lanes : HRP Anti-CD68 antibody [FA-11] (ab216526) at 1/5000 dilution

        Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
        Lane 2 : RAW 264.7 LPS treated (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 37 kDa
        Additional bands at: 100 kDa (possible glycosylated form)


        Exposure time: 20 minutes


        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab216526 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab216526? Please let us know so that we can cite the reference in this datasheet.

      ab216526 has not yet been referenced specifically in any publications.

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