Recombinant HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)
![Western blot - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)](https://www.abcam.com/ps/products/199/ab199203/Images/ab199203-252083-anti-cox1-cyclooxygenase-1-antibody-epr5866-hrp-western-blot.jpg "Western blot - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
-
Product name
HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
See all COX1 / Cyclooxygenase 1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: NIH 3T3, A431 and C2C12 whole cell lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 5.50 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5866 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
- Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199027)
- Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)
- PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (ab219375)
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 74 kDa (predicted molecular weight: 69 kDa).
|
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 74 kDa (predicted molecular weight: 69 kDa). |
Target
-
Function
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 5742 Human
- Entrez Gene: 19224 Mouse
- Entrez Gene: 24693 Rat
- Omim: 176805 Human
- SwissProt: P23219 Human
- SwissProt: P22437 Mouse
- SwissProt: Q63921 Rat
- Unigene: 201978 Human
see all -
Alternative names
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
Images
-
Western blot - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199203 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![Western blot - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)](https://www.abcam.com/ps/products/199/ab199203/Images/ab199203-252086-anti-cox1-cyclooxygenase-1-antibody-epr5866-hrp-western-blot.jpg)
Western blot - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199203 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![Western blot - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)](https://www.abcam.com/ps/products/199/ab199203/Images/ab199203-252084-anti-cox1-cyclooxygenase-1-antibody-epr5866-hrp-western-blot.jpg)
Western blot - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203) at 1/5000 dilution + C2C12 (Mouse skeletal muscle myoblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199203 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)](https://www.abcam.com/ps/products/199/ab199203/Images/ab199203-4-benefits-of-recombinant-antibodies.png)
HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab199203 has not yet been referenced specifically in any publications.