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    products/primary-antibodies/hrp-cytokeratin-19-antibody-ep1580y-ab193600.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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RecombinantRabMAb

Recombinant HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
  • Western blot - HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
  • HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1580Y] to Cytokeratin 19
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE Unconjugated

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Overview

  • Product name

    HRP Anti-Cytokeratin 19 antibody [EP1580Y]
    See all Cytokeratin 19 primary antibodies
  • Description

    HRP Rabbit monoclonal [EP1580Y] to Cytokeratin 19
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 cytoplasmic lysate. IHC: normal human colon tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 3.70 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.9% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1580Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
    • Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
    • Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
    • Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
    • Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
    • Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
    • APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
    • PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
    • Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Present in hair follicles at all stages of development.
  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Target information above from: UniProt accession P08727 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3880 Human
    • Entrez Gene: 16669 Mouse
    • Omim: 148020 Human
    • SwissProt: P08727 Human
    • SwissProt: P19001 Mouse
    • Unigene: 654568 Human
    • Unigene: 439699 Mouse
    • Alternative names

      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)

      IHC image of Cytokeratin 19 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193600 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
      Western blot - HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
      HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 44 kDa


      Exposure time: 5 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193600 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
      HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab193600? Please let us know so that we can cite the reference in this datasheet.

    ab193600 has not yet been referenced specifically in any publications.

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