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HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

Reviews (8) Submit a question References (65)
Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

Key features and details

  • HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies

  • Description

    HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute to 1.0 ml with distilled deionized water vortex gently and allow vial to sit on ice for 20 minutes. Store at +4°C. Do not freeze.

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.64% Sodium phosphate, 0.58% Sodium chloride, 1.5% BSA, 1.71% Sucrose
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    M2

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab49763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (7)
1/500 - 1/10000.
ELISA (1)
1/20000.
Notes
WB
1/500 - 1/10000.
ELISA
1/20000.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).

  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

Images

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview
    All lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) at 1/3000 dilution

    All lanes : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 45 seconds


    Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.

    5% milk blocking was used for 1 hour.

    See Abreview

  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    ELISA for the detection of FLAG®-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.

    Please note that in order to show a clearer image, we have brightened the original image.

    See Abreview

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    Saccharomyces cerevisiae purified FLAG®-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.

    See Abreview

References (65)

Publishing research using ab49763? Please let us know so that we can cite the reference in this datasheet.

ab49763 has been referenced in 65 publications.

  • Çaliskaner ZO  et al. Identification of novel TUBB1 variants in patients with macrothrombocytopenia. Turk J Med Sci 51:490-500 (2021). PubMed: 32892537
  • Zhao T  et al. Disome-seq reveals widespread ribosome collisions that promote cotranslational protein folding. Genome Biol 22:16 (2021). PubMed: 33402206
  • Yin XK  et al. PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer. Oncogene 40:1375-1389 (2021). PubMed: 33420374
  • Park JJ  et al. Checkpoint inhibition through small molecule-induced internalization of programmed death-ligand 1. Nat Commun 12:1222 (2021). PubMed: 33619272
  • Hannan FM  et al. Ap2s1 mutation causes hypercalcaemia in mice and impairs interaction between calcium-sensing receptor and adaptor protein-2. Hum Mol Genet 30:880-892 (2021). PubMed: 33729479
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 04 2014

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Purified protein (NIH3T3 cells)
Gel Running Conditions
Reduced Denaturing (3-8 % Tris-Acetate Gel)
Loading amount
5e+006 cells
Treatment
10 mM Sodium Butyrate for 24 hrs, 500 ug/ml Geneticin for 48 hrs
Specification
NIH3T3 cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 07 2011

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
2 µg
Specification
chimeric protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 17 2010

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (CHO-S, transfected with plasmid containing insert)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
125000 cells
Specification
CHO-S, transfected with plasmid containing insert
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 15 2010

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1 µg
Specification
BL21(DE3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 27 2010

ELISA abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Escherichia coli Purified protein (Mach1 (Invitrogen))
Specification
Mach1 (Invitrogen)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Type
Direct
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 19 2009

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Purified protein (BJ5453)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1 µg
Specification
BJ5453
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 14 2009

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (Mach1)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
0.01 µg
Specification
Mach1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 16 2008

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