HRP Anti-E tag antibody (ab19400)
Key features and details
- HRP Goat polyclonal to E tag
- Suitable for: ICC, ELISA, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-E tag antibody
See all E tag primary antibodies -
Description
HRP Goat polyclonal to E tag -
Host species
Goat -
Conjugation
HRP -
Tested applications
Suitable for: ICC, ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/200 - 1/500.
|
|
| ELISA |
1/10000 - 1/100000.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 1 kDa. Colorimetric: 1/1000 - 1/10000. Chemiluminesence: 1/1000 - 1/30000.
|
| Notes |
|---|
|
ICC
1/200 - 1/500. |
|
ELISA
1/10000 - 1/100000. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 1 kDa. Colorimetric: 1/1000 - 1/10000. Chemiluminesence: 1/1000 - 1/30000. |
Target
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Relevance
E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR. -
Alternative names
- E epitope tag antibody
- GAPVPYPDPLEPR epitope tag antibody
- GAPVPYPDPLEPR tag antibody
Images
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Western blot - HRP Anti-E tag antibody (ab19400)All lanes : HRP Anti-E tag antibody (ab19400) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 1 kDa
Exposure time: 3 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab19400 has been referenced in 5 publications.
- Cho A et al. Single-Cell Analysis Suggests that Ongoing Affinity Maturation Drives the Emergence of Pemphigus Vulgaris Autoimmune Disease. Cell Rep 28:909-922.e6 (2019). PubMed: 31340153
- Tung CP et al. Discovering neutralizing antibodies targeting the stem epitope of H1N1 influenza hemagglutinin with synthetic phage-displayed antibody libraries. Sci Rep 5:15053 (2015). PubMed: 26456860
- Isaacs AT et al. Engineered resistance to Plasmodium falciparum development in transgenic Anopheles stephensi. PLoS Pathog 7:e1002017 (2011). WB . PubMed: 21533066
- Mao X et al. Autoimmunity to desmocollin 3 in pemphigus vulgaris. Am J Pathol 177:2724-30 (2010). WB . PubMed: 20952584
- Xu L et al. Combinatorial surrobody libraries. Proc Natl Acad Sci U S A 105:10756-61 (2008). WB, ELISA . PubMed: 18664586